常规气相色谱法与快速气相色谱法在人血浆脂肪酸测定中的比较。
Comparison of conventional and fast gas chromatography in human plasma fatty acid determination.
作者信息
Bondia-Pons Isabel, Castellote Ana I, López-Sabater M Carmen
机构信息
Department de Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII s/n 08028, Barcelona, Spain.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Oct 5;809(2):339-44. doi: 10.1016/j.jchromb.2004.07.002.
A fast gas chromatographic technique for the determination of fatty acids in human plasma was developed. Its validation and comparison to a conventional method are here reported. The fast method significantly reduced the time required for analysis by a factor of 5 (total time of 3.2 min) while maintaining a similar resolution. Reproducibility of qualitative and quantitative data was measured in both applications. The results demonstrated that the applied fast gas chromatography (GC) conditions do not affect the analytical quality of the assays, allowing a short analysis time.
开发了一种用于测定人血浆中脂肪酸的快速气相色谱技术。本文报道了该技术的验证及其与传统方法的比较。快速方法在保持相似分辨率的同时，将分析所需时间显著缩短了5倍（总时间为3.2分钟）。在两种应用中都测定了定性和定量数据的重现性。结果表明，所应用的快速气相色谱（GC）条件不会影响测定的分析质量，从而实现了较短的分析时间。
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