Jones Taff, Cyr Sonya, Allard Francine, Bellerose Nathalie, Lowell George H, Burt David S
ID Biomedical Corporation of Quebec, 7150 Frederick Banting, Ville St-Laurent, Montreal, QC, Canada H4S 2A1.
Vaccine. 2004 Sep 9;22(27-28):3691-7. doi: 10.1016/j.vaccine.2004.03.035.
Protollin is a novel adjuvant comprising Proteosomes non-covalently complexed with LPS. Intranasal immunization of mice with Protollin combined with detergent-split influenza antigens (HA) or recombinant influenza hemagglutinin (rHA) enhanced serum IgG and mucosal IgA levels by up to 250-fold compared with immunization with the antigens alone. IFN-gamma responses were also enhanced compared to the levels produced by splenocytes from mice immunized with antigen alone, while production of IL-5 was abrogated. Mice immunized with Protollin-rHA were completely protected against lethal challenge with influenza virus, demonstrating that Protollin is an effective mucosal adjuvant for prophylactic vaccines.
Protollin是一种新型佐剂，由与脂多糖非共价复合的蛋白酶体组成。与单独用抗原免疫相比，用Protollin联合去污剂裂解流感抗原（HA）或重组流感血凝素（rHA）对小鼠进行鼻内免疫可使血清IgG和粘膜IgA水平提高多达250倍。与单独用抗原免疫的小鼠脾细胞产生的水平相比，IFN-γ反应也增强，而IL-5的产生被消除。用Protollin-rHA免疫的小鼠对流感病毒的致死性攻击具有完全的保护作用，表明Protollin是预防性疫苗的有效粘膜佐剂。
