Franssila Rauli, Hedman Klaus
Department of Virology, Haartman Institute and HUCH Diagnostic, University of Helsinki, P.O. Box 21 (Haarmaninkatu 3), Helsinki 00014, Finland.
Vaccine. 2004 Sep 9;22(27-28):3809-15. doi: 10.1016/j.vaccine.2003.06.003.
Recombinantly expressed virus-like particles of human parvovirus B19 containing the two structural proteins VP1 and VP2 (VP1/2 capsids) or VP2 alone (VP2 capsids) elicit vigorous antibody responses in animal models, whereas only VP1/2 capsids elicit neutralizing antibodies. VP1 is, therefore, essential for protective B-cell immunity. In this study, we determined the ability of VP1/2 capsids containing VP1 and VP2 in the ratio recommended for vaccine use, and of sole VP2 capsids to stimulate T-helper (Th) cells to proliferate and to secrete interferon gamma (IF-gamma) and interleukin-10 (IL-10) in humans long after natural infection. Similar proliferation, IF-gamma and IL-10 responses were found with the VP1/2 and VP2 capsids. We conclude that, whereas VP1 contains important B-cell epitopes, VP2, the major structural protein of human parvovirus B19, appears to provide the major target for B19-specific Th-cells years or decades after natural infection.
重组表达的包含两种结构蛋白VP1和VP2的人细小病毒B19病毒样颗粒（VP1/2衣壳）或仅VP2的病毒样颗粒（VP2衣壳）在动物模型中引发强烈的抗体反应，而只有VP1/2衣壳能引发中和抗体。因此，VP1对于保护性B细胞免疫至关重要。在本研究中，我们测定了含有按疫苗使用推荐比例的VP1和VP2的VP1/2衣壳以及单独的VP2衣壳在自然感染很久之后刺激人类辅助性T（Th）细胞增殖并分泌干扰素γ（IF-γ）和白细胞介素-10（IL-10）的能力。VP1/2衣壳和VP2衣壳产生了相似的增殖、IF-γ和IL-10反应。我们得出结论，虽然VP1含有重要的B细胞表位，但在自然感染数年或数十年后，人细小病毒B19的主要结构蛋白VP2似乎是B19特异性Th细胞的主要靶标。
