在人脐静脉内皮细胞存在的情况下凝血酶原的激活。
Activation of prothrombin in the presence of human umbilical-vein endothelial cells.
作者信息
Schoen P, Reutelingsperger C, Lindhout T
机构信息
Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Limburg, The Netherlands.
出版信息
Biochem J. 1992 Feb 1;281 ( Pt 3)(Pt 3):661-4. doi: 10.1042/bj2810661.
Abstract
Addition of Factor Xa, Factor Va and prothrombin to immobilized cultured human umbilical-vein endothelial cells resulted after a time delay in thrombin formation. The prothrombin-converting (prothrombinase) activity, however, was not associated with the cell surface. Rather, perturbation by thrombin, either formed in situ or exogenously added, induced a procoagulant phospholipid surface in the fluid phase, which, in the presence of Factor Xa and Factor Va, enabled the assembly of prothrombinase.
摘要
将凝血因子Xa、凝血因子Va和凝血酶原添加到固定化培养的人脐静脉内皮细胞中，经过一段时间延迟后会形成凝血酶。然而，凝血酶原转化（凝血酶原酶）活性与细胞表面无关。相反，由原位形成或外源添加的凝血酶引起的扰动会在液相中诱导促凝磷脂表面，在凝血因子Xa和凝血因子Va存在的情况下，该表面能够组装凝血酶原酶。
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