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本文引用的文献

1
Media conditioned by cultured human vascular endothelial cells inhibit the growth of vascular smooth muscle cells.由培养的人血管内皮细胞条件化的培养基抑制血管平滑肌细胞的生长。
Exp Cell Res. 1982 May;139(1):191-7. doi: 10.1016/0014-4827(82)90332-9.
2
Prothrombin is activated on vascular endothelial cells by factor Xa and calcium.凝血酶原在血管内皮细胞上被凝血因子Xa和钙离子激活。
Proc Natl Acad Sci U S A. 1983 Nov;80(22):7001-5. doi: 10.1073/pnas.80.22.7001.
3
Coagulation factor Va binds to human umbilical vein endothelial cells and accelerates protein C activation.凝血因子Va与人类脐静脉内皮细胞结合并加速蛋白C活化。
J Clin Invest. 1984 Jul;74(1):224-30. doi: 10.1172/JCI111405.
4
Preparation and stability of a highly purified human thrombin standard.高纯度人凝血酶标准品的制备与稳定性
Thromb Res. 1980 Oct 1;20(1):109-22. doi: 10.1016/0049-3848(80)90061-4.
5
Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.源自脐静脉的人内皮细胞培养。通过形态学和免疫学标准进行鉴定。
J Clin Invest. 1973 Nov;52(11):2745-56. doi: 10.1172/JCI107470.
6
Comparison of the esterase activities of trypsin, plasmin, and thrombin on guanidinobenzoate esters. Titration of the enzymes.胰蛋白酶、纤溶酶和凝血酶对胍基苯甲酸酯的酯酶活性比较。酶的滴定。
Biochemistry. 1969 May;8(5):2212-24. doi: 10.1021/bi00833a063.
7
Cellular processing of bovine factors X and Xa by cultured bovine aortic endothelial cells.培养的牛主动脉内皮细胞对牛因子X和Xa的细胞处理
J Exp Med. 1985 Aug 1;162(2):559-72. doi: 10.1084/jem.162.2.559.
8
Characterization of the interaction between factor Xa and bovine aortic endothelial cells.凝血因子Xa与牛主动脉内皮细胞之间相互作用的表征
Biochim Biophys Acta. 1985 Mar 21;844(3):320-9. doi: 10.1016/0167-4889(85)90133-8.
9
Isolation and partial purification of a novel anticoagulant from arteries of human umbilical cord.从人脐带动脉中分离并部分纯化一种新型抗凝剂。
Eur J Biochem. 1985 Sep 16;151(3):625-9. doi: 10.1111/j.1432-1033.1985.tb09150.x.
10
Enhancement by human umbilical vein endothelial cells of factor Xa-catalyzed activation of factor VII.人脐静脉内皮细胞对因子Xa催化的因子VII激活的增强作用。
Blood. 1988 Mar;71(3):791-6.

在人脐静脉内皮细胞存在的情况下凝血酶原的激活。

Activation of prothrombin in the presence of human umbilical-vein endothelial cells.

作者信息

Schoen P, Reutelingsperger C, Lindhout T

机构信息

Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Limburg, The Netherlands.

出版信息

Biochem J. 1992 Feb 1;281 ( Pt 3)(Pt 3):661-4. doi: 10.1042/bj2810661.

DOI:10.1042/bj2810661
PMID:1531589
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1130741/
Abstract

Addition of Factor Xa, Factor Va and prothrombin to immobilized cultured human umbilical-vein endothelial cells resulted after a time delay in thrombin formation. The prothrombin-converting (prothrombinase) activity, however, was not associated with the cell surface. Rather, perturbation by thrombin, either formed in situ or exogenously added, induced a procoagulant phospholipid surface in the fluid phase, which, in the presence of Factor Xa and Factor Va, enabled the assembly of prothrombinase.

摘要

将凝血因子Xa、凝血因子Va和凝血酶原添加到固定化培养的人脐静脉内皮细胞中，经过一段时间延迟后会形成凝血酶。然而，凝血酶原转化（凝血酶原酶）活性与细胞表面无关。相反，由原位形成或外源添加的凝血酶引起的扰动会在液相中诱导促凝磷脂表面，在凝血因子Xa和凝血因子Va存在的情况下，该表面能够组装凝血酶原酶。