Horré R, Schaal K P, Siekmeier R, Sterzik B, de Hoog G S, Schnitzler N
Institute for Medical Microbiology and Immunology, University of Bonn, Bonn, The Netherlands.
Respiration. 2004 Jul-Aug;71(4):360-6. doi: 10.1159/000079640.
Patients with cystic fibrosis (CF) are at an increased risk of pulmonary colonisation by opportunistic micro-organisms. Using specialised methods, the black yeast Exophiala dermatitidis could consistently be cultured from CF patients. Isolation rates from sputum samples ranged between 1.8 and 15.7%. Occasionally, infection could be recognised.
This study aimed at investigating the isolation rates of E. dermatitidis in samples taken from CF patients at the University of Bonn, Germany.
Altogether, 439 respiratory specimens taken from 81 CF patients were screened for the occurrence of E. dermatitidis over a period of 18 months. For the selective isolation of this fungus erythritol-chloramphenicol agar (ECA) produced in house was applied.
The isolation rate of E. dermatitidis was 1.1% from all specimens, 1.6% from all sputum samples and 6.2% in all patients examined.
Prior to the introduction of ECA, E. dermatitidis had never been isolated in our laboratory, either from CF, or from any other patient. During this study, E. dermatitidis was found to colonise the respiratory tract of some CF patients. The use of additional selective culture media is necessary for the recognition of uncommon fungi, e.g. E. dermatitidis, in CF patients.
囊性纤维化（CF）患者肺部被机会性微生物定植的风险增加。使用专门方法，可一直从CF患者中培养出黑色酵母菌皮炎外瓶霉。痰液样本的分离率在1.8%至15.7%之间。偶尔可识别出感染。
本研究旨在调查德国波恩大学CF患者样本中皮炎外瓶霉的分离率。
在18个月期间，对从81例CF患者采集的共439份呼吸道标本进行筛查，以检测皮炎外瓶霉的存在。为选择性分离这种真菌，应用了自制的赤藓醇 - 氯霉素琼脂（ECA）。
所有标本中皮炎外瓶霉的分离率为1.1%，所有痰液样本中为1.6%，所有接受检查的患者中为6.2%。
在引入ECA之前，我们实验室从未从CF患者或任何其他患者中分离出皮炎外瓶霉。在本研究期间，发现皮炎外瓶霉定植于一些CF患者的呼吸道。对于识别CF患者中不常见的真菌，如皮炎外瓶霉，使用额外的选择性培养基是必要的。
