Wilson W A, Clark M T, Pellmar T C
Science. 1977 Apr 22;196(4288):440-1. doi: 10.1126/science.15317.
The commonly used buffering agent tris(hydroxymethyl)methylamine (tris) antagonizes the action of iontophoretically applied acetylcholine on neurons of Aplysia californica. Concentrations of 5 to 10 millimolar tris markedly reduced both excitatory and inhibitory responses.
常用的缓冲剂三（羟甲基）甲胺（tris）可拮抗离子电渗法施加的乙酰胆碱对加州海兔神经元的作用。5至10毫摩尔的tris浓度可显著降低兴奋性和抑制性反应。
