Papaccio G, Esposito V
Institute of Anatomy, I School of Medicine, Naples, Italy.
Virchows Arch A Pathol Anat Histopathol. 1992;420(1):5-10. doi: 10.1007/BF01605977.
The aim of this study was to observe the ultrastructural events, during the onset of diabetes mellitus in the low-dose streptozocin (LDS)-treated mouse model with emphasis on the infiltrating elements. Forty male C57 BL/6J mice were given 40 mg/streptozocin on 5 consecutive days and killed 5, 6, 7, 8, 9, 10, 15, and 18 days after the first injection. Results demonstrated that islet infiltration occurring in LDS-treated mice is characterized by a very early pre-infiltration state in which mononuclear phagocytes in islet capillary vessels were considerably increased in number. A new histopathological time sequence for the early insulitis is described, in which attraction of blood mononuclear phagocytes into the islet capillary lumen is the first step. During the successive stage, occurring on days 6-8 we observed that mononuclear phagocytes migrate through capillary and venule walls into the islet parenchyma, where they differentiate into tissue macrophages. It was only later (step 3) that these macrophages acquired novel properties, typical of their "activated state" and started to phagocytose islet beta-cell debris. These data suggest that during the pre-infiltration and early insulitis the mononuclear phagocyte system plays a key role in the onset of LDS diabetes.
本研究的目的是观察低剂量链脲佐菌素（LDS）处理的小鼠模型中糖尿病发病过程中的超微结构变化，重点关注浸润成分。40只雄性C57 BL/6J小鼠连续5天每天给予40mg链脲佐菌素，并在首次注射后5、6、7、8、9、10、15和18天处死。结果表明，LDS处理的小鼠中发生的胰岛浸润的特征是一种非常早期的预浸润状态，其中胰岛毛细血管中的单核吞噬细胞数量显著增加。描述了早期胰岛炎的一种新的组织病理学时间顺序，其中血液单核吞噬细胞被吸引到胰岛毛细血管腔是第一步。在随后的第6-8天阶段，我们观察到单核吞噬细胞穿过毛细血管和小静脉壁迁移到胰岛实质，在那里它们分化为组织巨噬细胞。只是在后来（步骤3），这些巨噬细胞才获得了其“活化状态”典型的新特性，并开始吞噬胰岛β细胞碎片。这些数据表明，在预浸润和早期胰岛炎期间，单核吞噬细胞系统在LDS糖尿病的发病中起关键作用。
