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cDNA cloning for and preparation of antibodies against subunit d of H(+)-ATP synthase in rat mitochondria.

作者信息

Motojima K, Imanaka T

机构信息

Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan.

出版信息

Biochem Biophys Res Commun. 1992 Feb 14;182(3):1130-8. doi: 10.1016/0006-291x(92)91849-l.

DOI:10.1016/0006-291x(92)91849-l
PMID:1531750
Abstract

The cDNA for subunit d of rat mitochondrial H(+)-ATP synthase was cloned from a brain cDNA library. The protein contains internally repeat structures that have sequences similar to those of other ATP-related proteins. The antibodies raised against the protein A-subunit d fusion protein expressed in E. coli specifically recognized only the protein in the mitochondria. The Na2 CO3 fractionation followed by immunoblotting analyses suggest that at least a part of the protein is inserted into the membrane.

摘要

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