Higuti T, Osaka F, Yoshihara Y, Tsurumi C, Kawamura Y, Tani I, Toda H, Kakuno T, Sakiyama F, Tanaka K
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Biochem Biophys Res Commun. 1990 Sep 28;171(3):1079-86. doi: 10.1016/0006-291x(90)90794-n.
The nucleotide sequence of the import precursor of coupling factor 6 (factor 6) of rat liver H(+)-ATP synthase has been determined from a recombinant cDNA clone isolated by screening a rat liver cDNA library with a probe DNA. The sequence was composed of 458 nucleotides including a coding region for the import precursor of factor 6 and noncoding regions of both the 5'- and 3'-sides. The import precursor of factor 6 and its mature polypeptide deduced from the open reading frame consisted of 108 and 76 amino acid residues with a molecular weight of 12,494 and 8,927, respectively. The presequence of 32 amino acids could be the import signal peptide which serves to direct the protein into the mitochondrial matrix.
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