Su Chun-Lin, Chen Chun-Hung, Lu Hsin-Yi, Gean Po-Wu
Institute of Basci Medical Sciences and Department of Pharmacology, National Cheng-Kung University, Tainan, Taiwan.
Mol Pharmacol. 2004 Nov;66(5):1340-8. doi: 10.1124/mol.104.001156. Epub 2004 Aug 18.
Although the underlying mechanism is not elucidated, it has been postulated repeatedly that deprivation of sleep, particularly rapid eye movement (REM) sleep, affects learning. Here we report that memory for newly acquired information is impaired after a specific period of REM sleep deprivation (REMD). Memory retrieval-induced phosphorylation of protein kinases in the rat amygdala is abrogated by REMD that is associated with an increase in the expression of a dual protein/lipid phosphatase PTEN. REMD given before training is without effect, suggesting the lack of effect on the acquisition of memory. Intra-amygdala administration of antisense but not sense or scrambled oligonucleotides for PTEN prevents REMD-induced decrease in protein phosphorylation and impairment of fear memory. Thus, REMD interferes with the process of memory retention via the activation of PTEN.
尽管潜在机制尚未阐明，但人们反复推测，睡眠剥夺，尤其是快速眼动（REM）睡眠剥夺，会影响学习。在此我们报告，在特定时间段的REM睡眠剥夺（REMD）后，对新获取信息的记忆会受损。REMD消除了大鼠杏仁核中记忆检索诱导的蛋白激酶磷酸化，这与双蛋白/脂质磷酸酶PTEN表达增加有关。训练前进行REMD没有效果，表明对记忆获取没有影响。杏仁核内注射针对PTEN的反义寡核苷酸而非正义或随机寡核苷酸可防止REMD诱导的蛋白磷酸化减少和恐惧记忆受损。因此，REMD通过激活PTEN干扰记忆保持过程。
