Guzman-Marin Ruben, Suntsova Natalia, Bashir Tariq, Nienhuis Robert, Szymusiak Ronald, McGinty Dennis
Research Service, VA Greater Los Angeles Healthcare System, North Hills, CA 91343, USA.
Sleep. 2008 Feb;31(2):167-75. doi: 10.1093/sleep/31.2.167.
The dentate gyrus (DG) of the adult hippocampus contains progenitor cells, which have potential to differentiate into neurons. Previously we reported that 96 hours of total sleep deprivation reduces neurogenesis in the DG of adult rats. Loss of either non-rapid eye movement (NREM) or rapid eye movement (REM) sleep could have contributed to the effect of total sleep deprivation. The present study assessed the effect of 4 days of REM sleep deprivation (REMD) on neurogenesis.
REMD was achieved by brief treadmill movement initiated by automatic online detection of REM sleep. A yoked-control (YC) rat was placed in the same treadmill and experienced the identical movement regardless the stage of the sleep-wake cycle. The thymidine analog 5- bromo- 2'- deoxy-uridine and the intrinsic proliferation marker, Ki-67, were both used to label proliferating cells.
Basic neurophysiology laboratory.
Male Sprague-Dawley male rats (300-320 g).
REM sleep was reduced by 85% in REMD rats and by 43% in YC, compared with cage control animals and by 79% in REMD rats compared with YC. NREM sleep and slow wave activity within NREM did not differ in REMD and YC groups. Cell proliferation was reduced by 63 % in REMD compared with YC rats, and by 82% and 51%, respectively, in REMD and YC rats compared with cage controls. Across all animals, cell proliferation exhibited a positive correlation with the percentage of REM sleep (r = 0.84, P < 0.001). Reduced cell proliferation in REMD rats was confirmed with the intrinsic proliferation marker, Ki-67. REMD also reduced the percentage of proliferating cells that later expressed a mature neuronal marker.
The present findings support a hypothesis that REM sleep-associated processes facilitate proliferation of granule cells in the adult hippocampal DG.
成年海马体的齿状回（DG）含有祖细胞，这些祖细胞具有分化为神经元的潜力。此前我们报道，96小时的完全睡眠剥夺会减少成年大鼠齿状回中的神经发生。非快速眼动（NREM）睡眠或快速眼动（REM）睡眠的丧失都可能导致完全睡眠剥夺的这种影响。本研究评估了4天快速眼动睡眠剥夺（REMD）对神经发生的影响。
通过自动在线检测快速眼动睡眠启动的短暂跑步机运动来实现快速眼动睡眠剥夺。将一只配对对照（YC）大鼠置于同一跑步机上，无论其睡眠 - 觉醒周期处于何种阶段，都经历相同的运动。胸腺嘧啶类似物5 - 溴 - 2'- 脱氧尿苷和内在增殖标志物Ki - 67均用于标记增殖细胞。
基础神经生理学实验室。
雄性斯普拉格 - 道利大鼠（300 - 320克）。
与笼养对照动物相比，快速眼动睡眠剥夺组大鼠的快速眼动睡眠减少了85%，配对对照组减少了43%；与配对对照组相比，快速眼动睡眠剥夺组大鼠的快速眼动睡眠减少了79%。快速眼动睡眠剥夺组和配对对照组的非快速眼动睡眠及非快速眼动睡眠中的慢波活动没有差异。与配对对照大鼠相比，快速眼动睡眠剥夺组的细胞增殖减少了63%；与笼养对照相比，快速眼动睡眠剥夺组和配对对照组的细胞增殖分别减少了82%和51%。在所有动物中，细胞增殖与快速眼动睡眠百分比呈正相关（r = 0.84，P < 0.001）。用内在增殖标志物Ki - 67证实了快速眼动睡眠剥夺组大鼠细胞增殖减少。快速眼动睡眠剥夺还降低了后来表达成熟神经元标志物的增殖细胞百分比。
目前的研究结果支持这样一种假设，即与快速眼动睡眠相关的过程促进成年海马齿状回颗粒细胞的增殖。
