基于横向弛豫优化谱（TROSY）的核磁共振实验用于研究大分子动力学和氢键。

TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding.

作者信息

Zhu Guang, Xia Youlin, Lin Donghai, Gao Xiaolian

机构信息

Department of Biochemistry, The Hong Kong University of Science and Technology, Hong Kong, SAR People's Republic of China.

出版信息

Methods Mol Biol. 2004;278:161-84. doi: 10.1385/1-59259-809-9:161.

DOI:10.1385/1-59259-809-9:161

PMID:15317997

Abstract

Transverse relaxation-optimized spectroscopy (TROSY)-based nuclear magnetic resonance (NMR) experiments can be exploited to obtain chemical shift assignment and values of J-coupling constants, residual dipolar couplings, and nuclear Overhauser effects (NOEs) for structural studies of proteins, as discussed in Chapter 5. Furthermore, the application of TROSY-based NMR experiments can be extended to the measurements of molecule dynamics, amide proton exchange rates, and hydrogen bonds. This chapter describes these experiments.

摘要

基于横向弛豫优化谱（TROSY）的核磁共振（NMR）实验可用于获得化学位移归属以及J耦合常数、残余偶极耦合和核Overhauser效应（NOE）的值，以用于蛋白质的结构研究，如第5章所述。此外，基于TROSY的NMR实验的应用可扩展到分子动力学、酰胺质子交换率和氢键的测量。本章将描述这些实验。