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人类线粒体ATP合酶复合体δ亚基输入前体的分子克隆

Molecular cloning of an import precursor of the delta-subunit of the human mitochondrial ATP synthase complex.

作者信息

Jordan E M, Breen G A

机构信息

Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson 75083-0688.

出版信息

Biochim Biophys Acta. 1992 Feb 28;1130(1):123-6. doi: 10.1016/0167-4781(92)90477-h.

DOI:10.1016/0167-4781(92)90477-h
PMID:1531933
Abstract

Two types of cDNA clones encoding a precursor of the delta-subunit of the human mitochondrial F0F1 ATP synthase complex (EC 3.6.1.34) have been isolated from a human cDNA library. Both clones contain a 504 basepair open reading frame that encodes a polypeptide with a presequence 22 amino acids in length and a mature protein 146 residues in length. The difference between the two types of cDNA clones is the presence of a 296 basepair insert in the 3' untranslated region of the delta-subunit cDNA in one of the types.

摘要

从人cDNA文库中分离出了两种编码人线粒体F0F1 ATP合酶复合物(EC 3.6.1.34)δ亚基前体的cDNA克隆。两个克隆均含有一个504个碱基对的开放阅读框,该阅读框编码一个具有22个氨基酸长度的前序列和146个氨基酸长度的成熟蛋白的多肽。两种cDNA克隆之间的差异在于其中一种类型的δ亚基cDNA的3'非翻译区存在一个296个碱基对的插入片段。

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