Godfrey D I, Masciantonio M, Tucek C L, Malin M A, Boyd R L, Hugo P
Department of Pathology and Immunology, Monash University Medical School, Melbourne, Victoria, Australia.
J Immunol. 1992 Apr 1;148(7):2006-11.
In a previous study, we raised a mAb (MTS 35) reacting with a plasma membrane Ag expressed on both cortical thymocytes and a subset of thymic medullary epithelial cells. In view of the shared expression of this molecule, we have defined it as thymic shared Ag-1 (TSA-1). Considering its selective reactivity with cortical, but not medullary thymocytes, the relevance of TSA-1 as a marker of immature T cells was investigated in detail in this study, using multicolor flow cytometric analysis. TSA-1 was found on all immature thymocyte subsets (CD3-4-8-, CD3-4+8-, CD3-4-8+, CD3-4+8+, CD3low4+8+). Conversely, CD3high4+8- and CD3high4-8+ thymocytes, early thymic migrants and peripheral T cells were TSA-1-. More refined gating and analysis of the transitional CD3intermediate/high4+8+ thymocytes, proposed candidates for negative selection, demonstrated that approximately one half were TSA-1-. In fact, there was a directly inverse relationship between TSA-1 and CD3 expression on thymocytes. In the periphery, TSA-1 was detected on B lymphocytes. TSA-1 is PI-linked and has a molecular mass of 17 kDa nonreduced, or 12 to 13 kDa reduced. Through cross-correlation analysis, this molecule was distinct from H-2K, PNA-R, CD5, CD11a/18, Thy-1, HSA, Ly6A/E, Ly6C, ThB, CD25, CD44. Hence TSA-1 appears to be a unique marker which exquisitely separates mature from immature thymocytes.
在先前的一项研究中，我们制备了一种单克隆抗体（MTS 35），它能与皮质胸腺细胞和一部分胸腺髓质上皮细胞表面表达的一种质膜抗原发生反应。鉴于该分子的共同表达，我们将其定义为胸腺共享抗原-1（TSA-1）。考虑到其与皮质胸腺细胞而非髓质胸腺细胞的选择性反应性，本研究使用多色流式细胞术分析详细研究了TSA-1作为未成熟T细胞标志物的相关性。在所有未成熟胸腺细胞亚群（CD3-4-8-、CD3-4+8-、CD3-4-8+、CD3-4+8+、CD3low4+8+）上均发现了TSA-1。相反，CD3high4+8-和CD3high4-8+胸腺细胞、早期胸腺迁移细胞和外周T细胞均为TSA-1阴性。对过渡性CD3intermediate/high4+8+胸腺细胞（负向选择的候选细胞）进行更精细的门控和分析表明，大约一半为TSA-1阴性。事实上，胸腺细胞上TSA-1与CD3表达之间存在直接的负相关关系。在外周，在B淋巴细胞上检测到了TSA-1。TSA-1与磷脂酰肌醇相连，非还原状态下分子量为17 kDa，还原状态下为12至13 kDa。通过交叉相关分析，该分子与H-2K、PNA-R、CD5、CD11a/18、Thy-1、HSA、Ly6A/E、Ly6C、ThB、CD25、CD44不同。因此，TSA-1似乎是一种独特的标志物，能精确地区分成熟胸腺细胞和未成熟胸腺细胞。
