Hamanaka Yoshitaka, Numata Hideharu, Shiga Sakiko
Department of Bio- and Geosciences, Graduate School of Science, Osaka City University, Sumiyoshi, Osaka, 558-8585, Japan.
Cell Tissue Res. 2004 Nov;318(2):403-18. doi: 10.1007/s00441-004-0935-1. Epub 2004 Aug 19.
Morphological and electrical properties of neurons with somata in the pars intercerebralis (PI) and pars lateralis (PL) were examined by intracellular recording and staining in the adult blow fly, Protophormia terraenovae. According to the location of somata and fiber distribution, two types of PI neurons (PIa and PIb) and two types of PL neurons (PLa and PLb) were identified. PIb neurons were further divided into two subgroups of PIb1 and PIb2 depending on fiber branching patterns in the retrocerebral complex. PIa neurons projected axons to the contralateral nervi corporis cardiaci, whereas PLa and PLb neurons projected axons to the ipsilateral nervi corporis cardiaci. PIb neurons characteristically showed symmetrical morphology with their somata along the midline. PLb neurons had a large branching area in the subesophageal ganglion. In the retrocerebral complex, PIb2 and PLa neurons sent fibers into the corpus allatum. PIa, PIb1 and PLb neurons projected not to the corpus allatum but to the corpus cardiacum-hypocerebral complex or visceral muscles in their vicinity. PIa, PIb and PLa neurons showed long spike durations (3-10 ms). PLb neurons were immunoreactive with antisera against corazonin, FMRFamide, or beta-pigment-dispersing hormone. This is the first report revealing the morphology of individual neurons with somata residing in PI and PL in the adult fly.
通过对成年新陆原伏蝇（Protophormia terraenovae）进行细胞内记录和染色，研究了脑间部（PI）和外侧部（PL）中具有胞体的神经元的形态学和电学特性。根据胞体位置和纤维分布，鉴定出两种类型的PI神经元（PIa和PIb）和两种类型的PL神经元（PLa和PLb）。根据脑后复合体中的纤维分支模式，PIb神经元进一步分为PIb1和PIb2两个亚组。PIa神经元将轴突投射到对侧的心侧体神经，而PLa和PLb神经元将轴突投射到同侧的心侧体神经。PIb神经元的特征是其胞体沿中线呈现对称形态。PLb神经元在咽下神经节中有较大的分支区域。在脑后复合体中，PIb2和PLa神经元将纤维发送到咽侧体。PIa、PIb1和PLb神经元不投射到咽侧体，而是投射到心侧体-脑下复合体或其附近的内脏肌肉。PIa、PIb和PLa神经元显示出较长的峰电位持续时间（3-10毫秒）。PLb神经元与抗促心肽、FMRF酰胺或β-色素分散激素的抗血清发生免疫反应。这是首次报道揭示成年果蝇中位于PI和PL的具有胞体的单个神经元的形态。
