Hamanaka Yoshitaka, Tanaka Shinji, Numata Hideharu, Shiga Sakiko
Department of Biology and Geosciences, Graduate School of Science, Osaka City University, Sumiyoshi, Osaka 558-8585, Japan.
Cell Tissue Res. 2007 Sep;329(3):581-93. doi: 10.1007/s00441-007-0433-3. Epub 2007 Jun 5.
Antisera against a variety of vertebrate and invertebrate neuropeptides were used to characterize neurons with somata in the pars intercerebralis (PI), pars lateralis (PL), and subesophageal ganglion (SEG), designated as PI neurons, PL neurons, and SEG neurons, respectively, all of which project to the retrocerebral complex in the blow fly, Protophormia terraenovae. Immunocytochemistry combined with backfills through the cardiac-recurrent nerve revealed that at least two pairs of PI and SEG neurons for each were FMRFamide-immunoreactive. Immunoreactivity against [Arg7]-corazonin, beta-pigment-dispersing hormone (beta-PDH), cholecystokinin8, or FMRFamide was observed in PL neurons. Immunoreactive colocalization of [Arg7]-corazonin with beta-PDH, [Arg7]-corazonin with cholecystokinin8, or beta-PDH with FMRFamide was found in two to three somata in the PL of a hemisphere. Based on their anatomical and immunocytochemical characteristics, PI neurons were classified into two types, PL neurons into six types, and SEG neurons into two types. Fibers in the retrocerebral complex showed [Arg7]-corazonin, beta-PDH, cholecystokinin8, and FMRFamide immunoreactivity. Cholecystokinin8 immunoreactivity was also detected in intrinsic cells of the corpus cardiacum. The corpus allatum was densely innervated by FMRFamide-immunoreactive varicose fibers. These results suggest that PI, PL, and SEG neurons release [Arg7]-corazonin, beta-PDH, cholecystokinin8, or FMRFamide-like peptides from the corpus cardiacum or corpus allatum into the hemolymph, and that some PL neurons may simultaneously release several neuropeptides.
使用针对多种脊椎动物和无脊椎动物神经肽的抗血清来鉴定位于脑间部（PI）、外侧部（PL）和咽下神经节（SEG）中具有胞体的神经元，分别将其命名为PI神经元、PL神经元和SEG神经元，它们都投射到新陆原伏蝇的脑后复合体。免疫细胞化学结合通过心回神经的逆向填充显示，每类至少有两对PI和SEG神经元对FMRF酰胺具有免疫反应性。在PL神经元中观察到对[Arg7]-促前胸腺激素、β-色素分散激素（β-PDH）、胆囊收缩素8或FMRF酰胺的免疫反应性。在一个半球的PL中，两到三个胞体中发现了[Arg7]-促前胸腺激素与β-PDH、[Arg7]-促前胸腺激素与胆囊收缩素8或β-PDH与FMRF酰胺的免疫反应性共定位。根据其解剖学和免疫细胞化学特征，PI神经元分为两种类型，PL神经元分为六种类型，SEG神经元分为两种类型。脑后复合体中的纤维显示出[Arg7]-促前胸腺激素、β-PDH、胆囊收缩素8和FMRF酰胺免疫反应性。在心侧体的固有细胞中也检测到胆囊收缩素8免疫反应性。咽侧体被FMRF酰胺免疫反应性曲张纤维密集支配。这些结果表明，PI、PL和SEG神经元从心侧体或咽侧体向血淋巴中释放[Arg7]-促前胸腺激素、β-PDH、胆囊收缩素8或FMRF酰胺样肽，并且一些PL神经元可能同时释放几种神经肽。
