Hu Hua, Larson Ronald G
Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109-2136, USA.
Langmuir. 2004 Aug 31;20(18):7436-43. doi: 10.1021/la049423v.
We introduce a fast and simple one-step method, a variation of the methods of Barrett and Campbell and Bartlett, to synthesize monodisperse fluorescent particles that can be dispersed in organic solvents and have long excitation (649 nm) and emission wavelengths (679 nm). A lipophilic fluorescent dye, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate, is directly incorporated into PMMA particles through dispersion polymerization. A poly(hydroxystearic acid) graft (poly) methyl methacrylate (MM) and methacrylic acid (MA) copolymer is used as a stabilizer to prevent the particles from aggregating and flocculating in the nonaqueous solvents. The fluorescent PMMA particles are very uniform in size, bleach at very low rate, and behave like hard spheres in their ordering on substrates. One important achievement in our synthesis protocol is that we are able to produce particles of a desired size by choosing the composition of the reactants according to a predetermined relationship between particle size and composition of reactants. In addition, the effects of fluorescent dye and polar solvent (ethanol) on the formation and size of particles are discussed.
我们介绍了一种快速简便的一步法，它是巴雷特、坎贝尔和巴特利特方法的一种变体，用于合成可分散于有机溶剂中、具有长激发波长（649纳米）和发射波长（679纳米）的单分散荧光颗粒。一种亲脂性荧光染料，高氯酸1,1'-二十八烷基-3,3,3',3'-四甲基吲哚二碳菁，通过分散聚合直接掺入聚甲基丙烯酸甲酯颗粒中。聚（羟基硬脂酸）接枝的聚甲基丙烯酸甲酯与甲基丙烯酸共聚物用作稳定剂，以防止颗粒在非水溶剂中聚集和絮凝。荧光聚甲基丙烯酸甲酯颗粒尺寸非常均匀，漂白速率极低，并且在基底上排列时表现得像硬球。我们合成方案的一项重要成果是，我们能够根据颗粒尺寸与反应物组成之间的预定关系选择反应物组成，从而生产出所需尺寸的颗粒。此外，还讨论了荧光染料和极性溶剂（乙醇）对颗粒形成和尺寸的影响。
