Castagnaro M, Benatti A, Guarda F
Dipartimento di Patologia Animale, Facoltà di Medicina Veterinaria, Università degli Studi, Turin, Italy.
Res Vet Sci. 1992 Jan;52(1):120-2. doi: 10.1016/0034-5288(92)90071-9.
The progressive autolytic changes of atrial specific granules (ASGS) up to 24 hours after death were studied electron microscopically. Tissue samples taken from the left auricular myocardium of four pig hearts at 0, 0.5, 1.5, 3.5, 6, 12 and 24 hours post mortem were fixed for 24 hours by immersion in 2.5 per cent cacodylate buffered glutaraldehyde (pH 7.4). Two pig hearts were kept at 20 degrees C and two at 4 degrees C. Although typical autolytic changes were seen in many organelles, no remarkable modifications in aspect, distribution, content and diameter of ASGS were observed in all samples. The results of this study indicate that ASGS are not morphologically affected by the autolytic processes, at least during the first 24 hours. Hence, the electron microscopic evaluation of ASGS does not need a particular fixation method such as perfusion. This may eliminate technical problems when slaughterhouse material is used.
用电镜研究了死后24小时内心房特异性颗粒（ASGS）的进行性自溶变化。在死后0、0.5、1.5、3.5、6、12和24小时，从四颗猪心脏的左心耳心肌中取出组织样本，浸入2.5%的二甲胂酸钠缓冲戊二醛（pH 7.4）中固定24小时。两颗猪心脏保存在20摄氏度，两颗保存在4摄氏度。尽管在许多细胞器中都观察到了典型的自溶变化，但在所有样本中，ASGS的形态、分布、内容物和直径均未观察到明显改变。本研究结果表明，至少在最初的24小时内，ASGS在形态上不受自溶过程的影响。因此，对ASGS的电镜评估不需要特殊的固定方法，如灌注法。这可能消除使用屠宰场材料时的技术问题。
