Castagnaro M, Guarda F, Lucchi M L, Bortolami R
Dipartimento di Patologia Animale, Facoltà di Medicina Veterinaria, Università degli Studi di Torino, Italy.
Vet Res Commun. 1991;15(5):335-40. doi: 10.1007/BF00366989.
The atrial specific granules (ASGs) were studied in samples collected from the right and left auricles of conventionally slaughtered cows (10), pigs (16) and rabbits (8). In addition, the presence of atrial natriuretic factor (ANF) was detected by immunocytochemistry. Mature ASGs, characterized by the presence of highly osmiophilic and electron-dense material surrounded by a membrane, were present in all atrial myoendocrine cells and their diameters ranged from 100 to 470 nm in pigs, from 100 to 235 nm in cattle, and from 125 to 275 nm in rabbits. Immunoelectronmicroscopical studies revealed the presence of ANF in the ASGs of pigs and cattle, whereas anti-ANF polyclonal serum failed to detect any significative reaction in lapine ASGs. The ultrastructural features of the ASGs of pigs, cattle and rabbits described may be useful in comparing the morphological picture of several cardiac endocrine pathological conditions.
对从常规屠宰的奶牛（10头）、猪（16头）和兔子（8只）的右心耳和左心耳采集的样本中的心房特异性颗粒（ASGs）进行了研究。此外，通过免疫细胞化学检测心房利钠因子（ANF）的存在。成熟的ASGs以存在被膜包围的高度嗜锇性和电子致密物质为特征，存在于所有心房肌内分泌细胞中，其直径在猪中为100至470纳米，在牛中为100至235纳米，在兔子中为125至275纳米。免疫电子显微镜研究显示猪和牛的ASGs中存在ANF，而抗ANF多克隆血清未能在兔ASGs中检测到任何明显反应。所描述的猪、牛和兔子ASGs的超微结构特征可能有助于比较几种心脏内分泌病理状况的形态学情况。
