使用滤波对角化方法的快速3D核磁共振：应用于用13C标记的乙酰基衍生化的寡糖。

Rapid 3D NMR using the filter diagonalization method: application to oligosaccharides derivatized with 13C-labeled acetyl groups.

作者信息

Armstrong Geoffrey S, Cano Kristin E, Mandelshtam Vladimir A, Shaka A J, Bendiak Brad

机构信息

Chemistry Department, University of California, Irvine, CA 92697-2025, USA.

出版信息

J Magn Reson. 2004 Sep;170(1):156-63. doi: 10.1016/j.jmr.2004.06.002.

DOI:10.1016/j.jmr.2004.06.002

PMID:15324769

Abstract

Rapid 3D NMR spectroscopy of oligosaccharides having isotopically labeled acetyl "isotags" was made possible with high resolution in the indirect dimensions using the filter diagonalization method (FDM). A pulse sequence was designed for the optimal correlation of acetyl methyl protons, methyl carbons, and carbonyl carbons. The multi-dimensional nature of the FDM, coupled with the advantages of constant-time evolution periods, resulted in marked improvements over Fourier transform (FT) and mirror-image linear prediction (MI-LP) processing methods. The three methods were directly compared using identical data sets. A highly resolved 3D spectrum was achieved with the FDM using a very short experimental time (28 min).

摘要

利用滤波对角化方法（FDM），对带有同位素标记乙酰基“同位素标签”的寡糖进行快速三维核磁共振波谱分析，能够在间接维度上实现高分辨率。设计了一个脉冲序列，以实现乙酰基甲基质子、甲基碳和羰基碳的最佳关联。FDM的多维特性，再加上恒时演化期的优势，使得其相对于傅里叶变换（FT）和镜像线性预测（MI-LP）处理方法有了显著改进。使用相同的数据集对这三种方法进行了直接比较。使用FDM在非常短的实验时间（28分钟）内获得了高分辨率的三维谱图。