Rosenau Barbara J, Schur Peter H
Division of Rheumatology/Immunology/Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
J Autoimmun. 2004 Sep;23(2):179-82. doi: 10.1016/j.jaut.2004.05.005.
Serum amyloid A (SAA) is elevated in inflammatory states. While antibodies to CRP, the other major acute phase reactant, have been described, to our knowledge, antibodies to SAA have not. This study was therefore undertaken to determine whether antibodies to SAA could be detected in patients and whether these antibodies are associated with any disease, in particular inflammatory states, using CRP as a proxy for inflammation.
An ELISA technique was developed for the detection of antibodies to SAA. Specificity was demonstrated by inhibition. Serum from one hundred and thirty-eight patients sent to the Clinical Immunology Laboratory for CRP evaluations were tested for anti-SAA activity. Their charts were reviewed for clinical parameters, in particular for inflammation, to determine association. Patients were also divided into those with normal and elevated levels of CRP, as a proxy for inflammation. As a control we also studied 62 normals.
The mean OD and 3 SD of the 62 normal blood bank donors for anti-SAA was 0.411 +/- 0.577. Thus for the purposes of this study 0.988 was determined as the cutoff between normality and abnormality. Sixty-one normals and 114 patients had "normal" levels. Elevated levels were observed in 24 patients and 1 normal. There was an association between elevated levels and aortic stenosis, deep vein thrombosis, and systemic lupus erythematosus, seizures, stroke, and atrial fibrillation. There was no association between anti-SAA levels and CRP levels.
These results show that antibodies to SAA develop in some patients. There was some association with inflammatory cardiovascular diseases.
血清淀粉样蛋白A（SAA）在炎症状态下会升高。虽然针对另一种主要急性期反应物CRP的抗体已有报道，但据我们所知，针对SAA的抗体尚未见报道。因此，本研究旨在确定能否在患者中检测到抗SAA抗体，以及这些抗体是否与任何疾病相关，特别是炎症状态，以CRP作为炎症的替代指标。
开发了一种ELISA技术用于检测抗SAA抗体。通过抑制试验证明其特异性。对送往临床免疫实验室进行CRP评估的138例患者的血清进行抗SAA活性检测。查阅他们的病历以获取临床参数，特别是炎症参数，以确定相关性。患者还被分为CRP水平正常和升高的两组，作为炎症的替代指标。作为对照，我们还研究了62名正常人。
62名正常血库供血者的抗SAA平均光密度（OD）和3个标准差为0.411±0.577。因此，在本研究中，将0.988确定为正常与异常的临界值。61名正常人和114名患者的水平“正常”。24名患者和1名正常人的水平升高。升高水平与主动脉瓣狭窄、深静脉血栓形成、系统性红斑狼疮、癫痫发作、中风和心房颤动之间存在关联。抗SAA水平与CRP水平之间无关联。
这些结果表明，部分患者体内会产生抗SAA抗体。其与炎症性心血管疾病存在一定关联。
