Mills Marc A, Bonner James S, Page Cheryl A, Autenrieth Robin L
Department of Civil Engineering, Texas A&M University, College Station, TX 77843-3136, USA.
Mar Pollut Bull. 2004 Sep;49(5-6):425-35. doi: 10.1016/j.marpolbul.2004.02.027.
A controlled petroleum release was conducted to evaluate bioremediation in a wetland near Houston, Texas. The 140-day study was conducted using a randomized, complete block design to test three treatments with six replicates per treatment. The three treatment strategies were inorganic nutrients, inorganic nutrients with an alternative electron acceptor, and a no-action oiled control. Samples were analyzed for petroleum chemistry and inorganic nutrients. These results are discussed in the context of our related research involving toxicology and microbiology at the site during the experiment. To evaluate biodegradation, the targeted compounds were normalized to the conservative compound C3017alpha, 21beta-[H]hopane, thus reducing the effects of spatial heterogeneity and physical transport. The two biostimulation treatments demonstrated statistically-higher rates of biodegradation than the oiled no-action control. For the majority of the experiment, target nutrient levels were maintained. Further research may be warranted to optimize these bioremediation strategies as well as evaluating additional treatment strategies for wetlands and other shoreline systems.
在得克萨斯州休斯顿附近的一个湿地进行了一次受控石油泄漏，以评估生物修复效果。这项为期140天的研究采用随机完全区组设计，测试三种处理方法，每种处理方法有六个重复。三种处理策略分别是无机养分、添加替代电子受体的无机养分以及不采取行动的油污对照。对样品进行了石油化学和无机养分分析。在实验期间，结合我们在该地点进行的涉及毒理学和微生物学的相关研究对这些结果进行了讨论。为了评估生物降解情况，将目标化合物标准化为保守化合物C30 17α, 21β-[H]藿烷，从而减少空间异质性和物理迁移的影响。两种生物刺激处理方法的生物降解率在统计学上高于油污不采取行动的对照。在大部分实验中，目标养分水平得以维持。可能需要进一步研究来优化这些生物修复策略，以及评估针对湿地和其他海岸线系统的额外处理策略。
