Canada Nuno, Carvalheira Julio, Meireles Carla Sofia, Correia da Costa José Manuel, Rocha António
CECA, Center for the Study of Animal Sciences, Abel Salazar Institute of Biomedical Sciences, University of Porto, Campus Agrário de Vairão, R. Padre Armando Quintas, 4485-661 Vairão, Portugal.
Theriogenology. 2004 Oct 1;62(7):1229-35. doi: 10.1016/j.theriogenology.2004.01.004.
This study was carried out to determine the prevalence of neosporosis in an area of intensive dairy production, in Portugal. Sera samples were obtained in a random basis from 114 cows in 49 herds (group A), and from 1237 cows in 36 herds with a history of abortion outbreaks (group B). All sera samples were tested for neosporosis by direct agglutination test (DAT). Additionally, attempts to isolate Neospora caninum in 42 aborted bovine fetuses from 38 dairy herds (group C) were carried out, utilizing a bioassay with immuno-depressed Swiss Webster mice. Parasitological confirmation was done by indirect fluorescent antibody test (IFAT). The prevalence of neosporosis in the group A was 28%. Group B had a significantly (P < 0.001) higher prevalence (46%) and Neospora caninum was isolated in 36% of the aborted fetuses (group C). These results indicate that neosporosis, a disease only recently (2001) diagnosed in Portugal, has a high prevalence in the country, particularly in populations with a story of abortion. Thus, neosporosis should systematically be considered in the differential diagnosis of abortion. In the context of embryo transfers, the importance of selecting Neospora-free embryo recipients is discussed, as well as the pertinence of assessing the Neospora status of traded and imported cattle.
本研究旨在确定葡萄牙集约化奶牛养殖区新孢子虫病的流行情况。血清样本随机取自49个牛群中的114头奶牛（A组），以及36个有流产暴发史的牛群中的1237头奶牛（B组）。所有血清样本均通过直接凝集试验（DAT）检测新孢子虫病。此外，利用免疫抑制的瑞士韦伯斯特小鼠进行生物测定，对来自38个奶牛场的42头流产牛胎儿（C组）进行犬新孢子虫分离尝试。通过间接荧光抗体试验（IFAT）进行寄生虫学确认。A组新孢子虫病的患病率为28%。B组患病率显著更高（46%，P < 0.001），并且在36%的流产胎儿中分离出犬新孢子虫（C组）。这些结果表明，新孢子虫病这种在葡萄牙直到最近（2001年）才被诊断出的疾病，在该国具有高患病率，尤其是在有流产病史的牛群中。因此，在流产的鉴别诊断中应系统地考虑新孢子虫病。在胚胎移植的背景下，讨论了选择无新孢子虫胚胎受体的重要性，以及评估交易和进口牛的新孢子虫状况的相关性。
