McPherson Alexander
Department of Molecular Biology and Biochemistry, University of Califonia, Irvine 560 Steinhaus Hall, Irvine, CA 92697-3900, USA.
Methods. 2004 Nov;34(3):254-65. doi: 10.1016/j.ymeth.2004.03.019.
Biological macromolecules can be crystallized by a variety of techniques, and using a wide range of reagents which produce supersaturated mother liquors. These may, in turn, be applied under different physical conditions such as temperature. The fundamental approaches to devising successful crystallization conditions and the factors that influence them are summarized here. For the Novice, it is hoped that this brief review might serve as a useful introduction and a stepping-stone to a successful X-ray structure determination. In addition, it may provide a framework in which to place the articles that follow.
生物大分子可以通过多种技术进行结晶，并使用各种能产生过饱和母液的试剂。这些试剂又可以在不同的物理条件下（如温度）使用。本文总结了设计成功结晶条件的基本方法及其影响因素。对于新手而言，希望这篇简短的综述能作为有益的入门介绍，成为成功进行X射线结构测定的垫脚石。此外，它可能为后续文章提供一个框架。
