Imanaka T, Lazarow P B, Takano T
Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Biochim Biophys Acta. 1992 Apr 7;1134(3):197-202. doi: 10.1016/0167-4889(92)90176-c.
The inhibitory effect of suramin on the import of [35S]acyl-CoA oxidase into purified rat liver peroxisomes was investigated in vitro. The import of acyl-CoA oxidase was inhibited completely by 10 microM suramin, whilst the latency of catalase remained unchanged. The important value decreased 60% by pretreatment of peroxisomes with 10 microM suramin, but it did not decrease by pretreatment of translation products. Polysulfonate compounds which have two clusters of negative charges, such as Cibacron blue F3GA and Trypan blue, as well as suramin, inhibited the import, whilst mono- and disulfonate compounds did not.
在体外研究了苏拉明对[35S]酰基辅酶A氧化酶导入纯化大鼠肝脏过氧化物酶体的抑制作用。10微摩尔的苏拉明完全抑制了酰基辅酶A氧化酶的导入，而过氧化氢酶的潜伏性保持不变。用过氧化物酶体经10微摩尔苏拉明预处理后，重要值降低了60%，但翻译产物经预处理后并未降低。具有两簇负电荷的聚磺酸盐化合物，如汽巴蓝F3GA和锥虫蓝，以及苏拉明，均抑制导入，而单磺酸盐和二磺酸盐化合物则无此作用。
