Pardo-Mateos Almudena, Young C S H
Department of Microbiology, College of Physicians and Surgeons, Hammer Health Sciences Center, Columbia University, New York, NY 10032, USA.
Virology. 2004 Sep 15;327(1):50-9. doi: 10.1016/j.virol.2004.06.011.
Adenovirus IVa2 protein is essential and multifunctional, with roles in encapsidation and transcriptional activation of the Major Late Promoter (MLP), but the importance of the transcriptional function to viability has not been assessed. To address this question, viral genomes with multiple nonbinding mutations in the MLP downstream elements DE1 and DE2, alone or in combination with nonbinding mutations in the UPE (USF0), were constructed. The results show that DE1/2 and the UPE are functionally redundant, suggesting an important role of IVa2 protein in the activation of the MLP in vivo. Previously, a virus (vIVa2) expressing a 40-kDa IVa2 isoform was created. Neither the DE1/2 mutations nor the USF0 mutations could be recovered in this genetic background. These results suggest that this 40-kDa isoform can play a role in transcription.
腺病毒IVa2蛋白至关重要且具有多种功能，在病毒包装以及主要晚期启动子（MLP）的转录激活中发挥作用，但尚未评估其转录功能对病毒生存能力的重要性。为解决这个问题，构建了在MLP下游元件DE1和DE2中具有多个非结合突变的病毒基因组，这些突变单独存在或与上游启动元件（UPE，USF0）中的非结合突变组合存在。结果表明，DE1/2和UPE在功能上是冗余的，这表明IVa2蛋白在体内MLP的激活中起着重要作用。此前，构建了一种表达40 kDa IVa2异构体的病毒（vIVa2）。在这种遗传背景下，既无法恢复DE1/2突变，也无法恢复USF0突变。这些结果表明，这种40 kDa的异构体可能在转录中发挥作用。
