番茄TERF1通过激活下游基因的表达来调节乙烯反应并增强渗透胁迫耐受性。
Tomato TERF1 modulates ethylene response and enhances osmotic stress tolerance by activating expression of downstream genes.
作者信息
Huang Zejun, Zhang Zhijin, Zhang Xiuling, Zhang Hongbo, Huang Dafang, Huang Rongfeng
机构信息
National Plant Gene Research Center (Beijing), Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
出版信息
FEBS Lett. 2004 Aug 27;573(1-3):110-6. doi: 10.1016/j.febslet.2004.07.064.
The interaction between ethylene and osmotic stress pathways modulates the expression of the genes relating to stress adaptation; however, the mechanism is not well understood. In this paper, we report a novel ethylene responsive factor, tomato ethylene responsive factor 1 (TERF1), that integrates ethylene and osmotic stress pathways. Biochemical analysis indicated that TERF1 binds to the GCC box (an element responsive to ethylene) and to the dehydration responsive element, which is responsive to the osmoticum. Expression of TERF1 was induced by ethylene and NaCl treatment. Under normal growth conditions, overexpression of TERF1 in tobacco activated the expression of GCC box-containing pathogen related genes and also caused the typical ethylene triple response. Further investigation indicated that transgenic TERF1 tobacco exhibited salt tolerance, suggesting that TERF1 might function as a linker between the ethylene and osmotic stress pathways.
乙烯与渗透胁迫途径之间的相互作用调节与胁迫适应相关基因的表达；然而，其机制尚未完全清楚。在本文中，我们报道了一种新型乙烯响应因子——番茄乙烯响应因子1（TERF1），它整合了乙烯和渗透胁迫途径。生化分析表明，TERF1与GCC框（一种对乙烯响应的元件）以及对渗透压响应的脱水响应元件结合。TERF1的表达受乙烯和NaCl处理诱导。在正常生长条件下，烟草中TERF1的过表达激活了含GCC框的病程相关基因的表达，还引发了典型的乙烯三重反应。进一步研究表明，转基因TERF1烟草表现出耐盐性，这表明TERF1可能作为乙烯和渗透胁迫途径之间的连接物发挥作用。
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