Li Yanwei, Wang Ying, Wu Xinyi, Wang Jian, Wu Xiaohua, Wang Baogen, Lu Zhongfu, Li Guojing
Institute of Vegetables, State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Front Plant Sci. 2021 May 7;12:650157. doi: 10.3389/fpls.2021.650157. eCollection 2021.
wilt (FW) is a typical soil-borne disease that seriously affects the yield and fruit quality of bottle gourd. Thus, to improve resistance to FW in bottle gourd, the genetic mechanism underlying FW resistance needs to be explored. In this study, we performed a genome-wide association study (GWAS) based on 5,330 single-nucleotide polymorphisms (SNPs) and 89 bottle gourd accessions. The GWAS results revealed a total of 10 SNPs ( ≤ 0.01, -log ≥ 2.0) significantly associated with FW resistance that were detected in at least two environments (2019DI, 2020DI, and the average across the 2 years); these SNPs were located on chromosomes 1, 2, 3, 4, 8, and 9. Linkage disequilibrium (LD) block structure analysis predicted three potential candidate genes for FW resistance. Genes and were within the range of the mean LD block of the marker BGReSe_14202; gene was 280 kb upstream of the marker BGReSe_00818. Real-time quantitative PCR (qRT-PCR) analysis showed that was significantly up-regulated in FW-infected plants of YD-4, Yin-10, and Hanbi; and were specifically up-regulated in FW-infected plants of YD-4. Therefore, gene is likely the major effect candidate gene for resistance against FW in bottle gourd. This work provides scientific evidence for the exploration of candidate gene and development of functional markers in FW-resistant bottle gourd breeding programs.
枯萎病（FW）是一种典型的土传病害，严重影响瓠瓜的产量和果实品质。因此，为提高瓠瓜对枯萎病的抗性，需要探索其抗性的遗传机制。在本研究中，我们基于5330个单核苷酸多态性（SNP）和89份瓠瓜种质进行了全基因组关联研究（GWAS）。GWAS结果显示，共有10个SNP（≤0.01，-log≥2.0）与枯萎病抗性显著相关，这些SNP在至少两个环境（2019DI、2020DI以及两年的平均值）中被检测到；它们位于第1、2、3、4、8和9号染色体上。连锁不平衡（LD）块结构分析预测了三个潜在的枯萎病抗性候选基因。基因 和 在标记BGReSe_14202的平均LD块范围内；基因 在标记BGReSe_00818上游280 kb处。实时定量PCR（qRT-PCR）分析表明， 在YD-4、Yin-10和Hanbi的枯萎病感染植株中显著上调； 和 在YD-4的枯萎病感染植株中特异性上调。因此，基因 可能是瓠瓜抗枯萎病的主要效应候选基因。这项工作为抗枯萎病瓠瓜育种计划中候选基因的探索和功能标记的开发提供了科学依据。
