Weldhagen Gerhard F
Department of Medical Microbiology, Pathology, Faculty of Health Sciences, University of Pretoria, P.O. Box 2034, Pretoria 0001, South Africa.
Antimicrob Agents Chemother. 2004 Sep;48(9):3402-6. doi: 10.1128/AAC.48.9.3402-3406.2004.
Extended-spectrum beta-lactamases (ESBLs) in Pseudomonas aeruginosa, such as GES-2, which compromises the efficacy of imipenem, tend to be geographically restricted. The CC-to-AA base pair substitution at positions 493 and 494 of the bla(GES-2)-coding region distinguishes this ESBL from bla(GES-1) and the bla(IBC)-type genes, making it an ideal target for the development of a novel sequence-specific, peptide nucleic acid (PNA)-based multiplex PCR detection method. By using two primer pairs in conjunction with a PNA probe, this method provided an accurate means of identification of bla(GES-2) compared to standard PCR and gene sequencing techniques when it was used to test 100 P. aeruginosa clinical isolates as well as previously published, well-described control strains encompassing all presently known genes in the bla(GES-IBC) ESBL family. This novel method has the potential to be used in large-scale, cost-effective screening programs for specific or geographically restricted ESBLs.
铜绿假单胞菌中的超广谱β-内酰胺酶（ESBLs），如GES-2，会降低亚胺培南的疗效，且往往具有地域局限性。bla(GES-2)编码区第493和494位的CC到AA碱基对替换将这种ESBL与bla(GES-1)及bla(IBC)型基因区分开来，使其成为开发一种新型的基于序列特异性肽核酸（PNA）的多重PCR检测方法的理想靶点。通过使用两对引物与一个PNA探针相结合，当该方法用于检测100株铜绿假单胞菌临床分离株以及先前发表的、涵盖bla(GES-IBC) ESBL家族所有已知基因的详细对照菌株时，与标准PCR和基因测序技术相比，它提供了一种准确鉴定bla(GES-2)的方法。这种新方法有可能用于针对特定或地域受限的ESBLs的大规模、经济高效的筛查项目。
