• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用实时荧光定量聚合酶链反应检测及序列特异性鉴别铜绿假单胞菌中的超广谱β-内酰胺酶GES-2

Rapid detection and sequence-specific differentiation of extended-spectrum beta-lactamase GES-2 from Pseudomonas aeruginosa by use of a real-time PCR assay.

作者信息

Weldhagen Gerhard F

机构信息

Mailing address: Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, P.O. Box 2034, Pretoria, 0001, South Africa.

出版信息

Antimicrob Agents Chemother. 2004 Oct;48(10):4059-62. doi: 10.1128/AAC.48.10.4059-4062.2004.

DOI:10.1128/AAC.48.10.4059-4062.2004
PMID:15388481
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC521898/
Abstract

The LightCycler was compared to nested PCR for the detection of bla(GES/IBC) genes from 100 Pseudomonas aeruginosa clinical isolates. The real-time PCR assay detected a bla(GES/IBC) gene product from 83 isolates, exhibiting a sensitivity and specificity of 94.3 and 100% respectively, compared to nested PCR and DNA sequencing.

摘要

将LightCycler与巢式PCR进行比较，以检测100株铜绿假单胞菌临床分离株中的bla(GES/IBC)基因。与巢式PCR和DNA测序相比，实时PCR检测法从83株分离株中检测到bla(GES/IBC)基因产物，其灵敏度和特异性分别为94.3%和100%。

相似文献

1
Rapid detection and sequence-specific differentiation of extended-spectrum beta-lactamase GES-2 from Pseudomonas aeruginosa by use of a real-time PCR assay.利用实时荧光定量聚合酶链反应检测及序列特异性鉴别铜绿假单胞菌中的超广谱β-内酰胺酶GES-2
Antimicrob Agents Chemother. 2004 Oct;48(10):4059-62. doi: 10.1128/AAC.48.10.4059-4062.2004.
2
Sequence-selective recognition of extended-spectrum beta-lactamase GES-2 by a competitive, peptide nucleic acid-based multiplex PCR assay.通过竞争性基于肽核酸的多重聚合酶链反应检测法对超广谱β-内酰胺酶GES-2进行序列选择性识别。
Antimicrob Agents Chemother. 2004 Sep;48(9):3402-6. doi: 10.1128/AAC.48.9.3402-3406.2004.
3
Emergence of class 1 integron-associated GES-5 and GES-5-like extended-spectrum beta-lactamases in clinical isolates of Pseudomonas aeruginosa in South Africa.南非铜绿假单胞菌临床分离株中与1类整合子相关的GES-5和GES-5样超广谱β-内酰胺酶的出现。
Int J Antimicrob Agents. 2008 Jun;31(6):527-30. doi: 10.1016/j.ijantimicag.2008.01.020. Epub 2008 Apr 23.
4
A Pseudomonas aeruginosa isolate producing the GES-5 extended-spectrum beta-lactamase.一株产GES-5型超广谱β-内酰胺酶的铜绿假单胞菌分离株。
J Antimicrob Chemother. 2006 Jun;57(6):1261-2. doi: 10.1093/jac/dkl116. Epub 2006 Apr 14.
5
Quantitative detection of metallo-beta-lactamase of blaIMP-cluster-producing Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis for rapid diagnosis and treatment of nosocomial infection.通过实时聚合酶链反应结合熔解曲线分析对产blaIMP基因簇的铜绿假单胞菌金属β-内酰胺酶进行定量检测，以快速诊断和治疗医院感染。
Diagn Microbiol Infect Dis. 2008 Jun;61(2):222-6. doi: 10.1016/j.diagmicrobio.2008.01.018. Epub 2008 Mar 17.
6
Novel variant (bla(VIM-11)) of the metallo-{beta}-lactamase bla(VIM) family in a GES-1 extended-spectrum-{beta}-lactamase-producing Pseudomonas aeruginosa clinical isolate in Argentina.在阿根廷一株产GES-1超广谱β-内酰胺酶的铜绿假单胞菌临床分离株中发现金属β-内酰胺酶bla(VIM)家族的新型变体（bla(VIM-11)）
Antimicrob Agents Chemother. 2005 Jan;49(1):474-5. doi: 10.1128/AAC.49.1.474-475.2005.
7
Detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients in Ahwaz, Iran.伊朗阿瓦士烧伤患者中分离出的产金属β-内酰胺酶铜绿假单胞菌菌株的检测
Diagn Microbiol Infect Dis. 2008 Jan;60(1):125-8. doi: 10.1016/j.diagmicrobio.2007.08.003. Epub 2007 Sep 27.
8
Molecular detection of GES-2 extended spectrum Beta-lactamase producing Pseudomonas aeruginosa in Pretoria, South Africa.南非比勒陀利亚产GES-2型超广谱β-内酰胺酶铜绿假单胞菌的分子检测
Int J Antimicrob Agents. 2004 Jul;24(1):35-8. doi: 10.1016/j.ijantimicag.2003.12.012.
9
OXA-type beta-lactamases among extended-spectrum cephalosporin-resistant Pseudomonas aeruginosa isolates in a university hospital in southern Taiwan.台湾南部一所大学医院中耐广谱头孢菌素的铜绿假单胞菌分离株中的OXA型β-内酰胺酶
J Microbiol Immunol Infect. 2006 Apr;39(2):130-4.
10
[Identification of SHV-type extended spectrum beta-lactamase genes in Pseudomonas aeruginosa by PCR-restriction fragment length polymorphism and insertion site restriction-PCR].[通过聚合酶链反应-限制性片段长度多态性和插入位点限制性聚合酶链反应鉴定铜绿假单胞菌中的SHV型超广谱β-内酰胺酶基因]
Pathol Biol (Paris). 2009 Jul;57(5):420-4. doi: 10.1016/j.patbio.2008.07.024. Epub 2008 Oct 5.

引用本文的文献

1
Antimicrobial Resistance Patterns and Prevalence of blaPER-1 and blaVEB-1 Genes Among ESBL-producing Pseudomonas aeruginosa Isolates in West of Iran.伊朗西部产超广谱β-内酰胺酶的铜绿假单胞菌分离株的耐药模式及blaPER-1和blaVEB-1基因的流行情况
Jundishapur J Microbiol. 2014 Jan;7(1):e8888. doi: 10.5812/jjm.8888. Epub 2014 Jan 1.
2
Molecular analysis of population structure and antibiotic resistance of Klebsiella isolates from a three-year surveillance program in Florence hospitals, Italy.意大利佛罗伦萨医院三年监测项目中分离的肺炎克雷伯菌的种群结构和抗生素耐药性的分子分析。
Eur J Clin Microbiol Infect Dis. 2012 Mar;31(3):371-8. doi: 10.1007/s10096-011-1319-6. Epub 2011 Jul 9.
3
BEL-2, an extended-spectrum beta-lactamase with increased activity toward expanded-spectrum cephalosporins in Pseudomonas aeruginosa.铜绿假单胞菌中对扩展谱头孢菌素具有更高活性的超广谱β-内酰胺酶 BEL-2。
Antimicrob Agents Chemother. 2010 Jan;54(1):533-5. doi: 10.1128/AAC.00859-09. Epub 2009 Nov 2.
4
Identification of CTX-M-type extended-spectrum-beta-lactamase genes using real-time PCR and pyrosequencing.使用实时聚合酶链反应和焦磷酸测序法鉴定CTX-M型超广谱β-内酰胺酶基因
Antimicrob Agents Chemother. 2007 Jan;51(1):223-30. doi: 10.1128/AAC.00611-06. Epub 2006 Nov 6.
5
Detection of extended-spectrum beta-lactamases in clinical isolates of Pseudomonas aeruginosa.铜绿假单胞菌临床分离株中广谱β-内酰胺酶的检测
Antimicrob Agents Chemother. 2006 Sep;50(9):2990-5. doi: 10.1128/AAC.01511-05.
6
Pyrosequencing as a rapid tool for identification of GES-type extended-spectrum beta-lactamases.焦磷酸测序法作为鉴定GES型超广谱β-内酰胺酶的快速工具。
J Clin Microbiol. 2006 Aug;44(8):3008-11. doi: 10.1128/JCM.02576-05.

本文引用的文献

1
Sequence-selective recognition of extended-spectrum beta-lactamase GES-2 by a competitive, peptide nucleic acid-based multiplex PCR assay.通过竞争性基于肽核酸的多重聚合酶链反应检测法对超广谱β-内酰胺酶GES-2进行序列选择性识别。
Antimicrob Agents Chemother. 2004 Sep;48(9):3402-6. doi: 10.1128/AAC.48.9.3402-3406.2004.
2
Integrons and beta-lactamases--a novel perspective on resistance.整合子与β-内酰胺酶——耐药性的新视角
Int J Antimicrob Agents. 2004 Jun;23(6):556-62. doi: 10.1016/j.ijantimicag.2004.03.007.
3
Emergence of the extended-spectrum beta-lactamase GES-1 in a Pseudomonas aeruginosa strain from Brazil: report from the SENTRY antimicrobial surveillance program.巴西一株铜绿假单胞菌中出现超广谱β-内酰胺酶GES-1：哨兵抗菌监测项目报告
Antimicrob Agents Chemother. 2004 Jun;48(6):2344-5. doi: 10.1128/AAC.48.6.2344-2345.2004.
4
Nosocomial spread of ceftazidime-resistant Klebsiella pneumoniae strains producing a novel class a beta-lactamase, GES-3, in a neonatal intensive care unit in Japan.在日本一家新生儿重症监护病房中，产新型A类β-内酰胺酶GES-3的耐头孢他啶肺炎克雷伯菌菌株的医院内传播。
Antimicrob Agents Chemother. 2004 Jun;48(6):1960-7. doi: 10.1128/AAC.48.6.1960-1967.2004.
5
Impact of DNA polymerases and their buffer systems on quantitative real-time PCR.DNA聚合酶及其缓冲系统对定量实时聚合酶链反应的影响
J Clin Microbiol. 2004 Jan;42(1):408-11. doi: 10.1128/JCM.42.1.408-411.2004.
6
Drug resistance genes and trailing growth in Candida albicans isolates.白色念珠菌分离株中的耐药基因与拖尾生长
J Antimicrob Chemother. 2004 Feb;53(2):217-24. doi: 10.1093/jac/dkh040. Epub 2003 Dec 19.
7
Ambler class A extended-spectrum beta-lactamases in Pseudomonas aeruginosa: novel developments and clinical impact.铜绿假单胞菌中的安布勒A类超广谱β-内酰胺酶：新进展与临床影响
Antimicrob Agents Chemother. 2003 Aug;47(8):2385-92. doi: 10.1128/AAC.47.8.2385-2392.2003.
8
An integron-associated beta-lactamase (IBC-2) from Pseudomonas aeruginosa is a variant of the extended-spectrum beta-lactamase IBC-1.来自铜绿假单胞菌的一种整合子相关β-内酰胺酶（IBC-2）是超广谱β-内酰胺酶IBC-1的一个变体。
J Antimicrob Chemother. 2001 Nov;48(5):627-30. doi: 10.1093/jac/48.5.627.
9
Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat.21世纪的超广谱β-内酰胺酶：这种重要耐药威胁的特征、流行病学及检测
Clin Microbiol Rev. 2001 Oct;14(4):933-51, table of contents. doi: 10.1128/CMR.14.4.933-951.2001.
10
GES-2, a class A beta-lactamase from Pseudomonas aeruginosa with increased hydrolysis of imipenem.GES-2，一种来自铜绿假单胞菌的A类β-内酰胺酶，对亚胺培南的水解能力增强。
Antimicrob Agents Chemother. 2001 Sep;45(9):2598-603. doi: 10.1128/AAC.45.9.2598-2603.2001.