利用实时荧光定量聚合酶链反应检测及序列特异性鉴别铜绿假单胞菌中的超广谱β-内酰胺酶GES-2
Rapid detection and sequence-specific differentiation of extended-spectrum beta-lactamase GES-2 from Pseudomonas aeruginosa by use of a real-time PCR assay.
作者信息
Weldhagen Gerhard F
机构信息
Mailing address: Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, P.O. Box 2034, Pretoria, 0001, South Africa.
出版信息
Antimicrob Agents Chemother. 2004 Oct;48(10):4059-62. doi: 10.1128/AAC.48.10.4059-4062.2004.
Abstract
The LightCycler was compared to nested PCR for the detection of bla(GES/IBC) genes from 100 Pseudomonas aeruginosa clinical isolates. The real-time PCR assay detected a bla(GES/IBC) gene product from 83 isolates, exhibiting a sensitivity and specificity of 94.3 and 100% respectively, compared to nested PCR and DNA sequencing.
摘要
将LightCycler与巢式PCR进行比较，以检测100株铜绿假单胞菌临床分离株中的bla(GES/IBC)基因。与巢式PCR和DNA测序相比，实时PCR检测法从83株分离株中检测到bla(GES/IBC)基因产物，其灵敏度和特异性分别为94.3%和100%。
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