Sakuma M, Yamada J, Suga T
Department of Clinical Biochemistry, Tokyo College of Pharmacy, Japan.
Biochem Pharmacol. 1992 Mar 17;43(6):1269-73. doi: 10.1016/0006-2952(92)90502-a.
The in-vivo effect of dehydroepiandrosterone (DHEA) on hepatic enzyme activities of rats, mice, hamsters and guinea pigs was investigated. After DHEA treatment (300 mg/kg body weight, per os, 14 days), the activities of peroxisomal beta-oxidation, catalase, carnitine acetyltransferase, carnitine palmitoyltransferase, lauric acid omega-hydroxylation, 1-acylglycerophosphocholine acyltransferase, malic enzyme and cytosolic palmitoyl-CoA hydrolase were increased in rats and in mice although to a smaller extent in the latter. These enzyme activities, however, were unchanged in hamsters with the exception of omega-hydroxylation (2.5-fold increase) and 1-acylglycerophosphocholine acyltransferase (2.0-fold increase). No significant changes were observed in any of these enzyme activities in guinea pigs. Immunoblot analysis confirmed the induction of peroxisomal acyl-CoA oxidase and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme in rats and mice. These results indicate that there are species differences in the inducing effect of DHEA on hepatic peroxisome proliferation-associated enzymes, which correlates well with the enzyme induction observed with other peroxisome proliferators.
研究了脱氢表雄酮（DHEA）对大鼠、小鼠、仓鼠和豚鼠肝脏酶活性的体内作用。给予DHEA处理（300mg/kg体重，经口给药，14天）后，大鼠和小鼠体内过氧化物酶体β-氧化、过氧化氢酶、肉碱乙酰转移酶、肉碱棕榈酰转移酶、月桂酸ω-羟化酶、1-酰基甘油磷酸胆碱酰基转移酶、苹果酸酶和胞质棕榈酰辅酶A水解酶的活性增加，不过小鼠增加的程度较小。然而，除了ω-羟化酶（增加2.5倍）和1-酰基甘油磷酸胆碱酰基转移酶（增加2.0倍）外，仓鼠的这些酶活性没有变化。豚鼠的这些酶活性均未观察到显著变化。免疫印迹分析证实了大鼠和小鼠体内过氧化物酶体酰基辅酶A氧化酶和烯酰辅酶A水合酶/3-羟酰基辅酶A脱氢酶双功能酶的诱导。这些结果表明，DHEA对肝脏过氧化物酶体增殖相关酶的诱导作用存在种属差异，这与其他过氧化物酶体增殖剂所观察到的酶诱导作用密切相关。
