携带蜂毒肽基因的重组腺病毒对肝癌细胞的诱导凋亡作用

[The induced apoptosis of recombinant adenovirus carrying melittin gene for hepatocellular carcinoma cell].

作者信息

Li Bai, Ling Chang-quan, Zhang Chen, Gu Wei, Li Shao-xiang, Huang Xue-qiang, Zhang Ya-ni, Yu Chao-qin

机构信息

Department of Chinese Traditional Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2004 Aug;12(8):453-5.

PMID:15329201

Abstract

OBJECTIVE

To observe the induced apoptosis of recombinant adenovirus carrying melittin gene (Ad-rAFP-Mel) for hepatocellular carcinoma cell line (BEL-7402).

METHODS

The morphological observe, DNA electrophoresis, TUNEL and Flow cytometry assay were used to study the apoptosis of BEL-7042 cell line transfected by Ad-rAFP-Mel.

RESULTS

The morphological changes and apoptosis of BEL-7402 transfected by Ad-rAFP-Mel were confirmed with microscopy and DNA electrophoresis, TUNEL, Flow cytometry assay. The DNA ladder could be demonstrated on DNA electrophoresis in Ad-rAFP-Mel group. The apoptosis rates of BEL-7402 cells in Ad-rAFP-Mel, Ad-rAFP, and control groups were (21.5+/-2.4)%, (10.5+/-4.4)% and (3.0+/-1.4)% respectively by TUNEL assay (F = 38.0, P < 0.05) and were (7.3+/-0.5)%, (3.9+/-0.1)% and (0.8+/-0.1)% respectively by flow cytometry assay (F = 415.1, P < 0.05).

CONCLUSION

It seems that melittin inducing apoptosis might be one of the antitumor mechanisms.

摘要

目的

观察携带蜂毒肽基因的重组腺病毒（Ad-rAFP-Mel）对肝癌细胞系（BEL-7402）的诱导凋亡作用。

方法

采用形态学观察、DNA电泳、TUNEL法及流式细胞术检测Ad-rAFP-Mel转染BEL-7042细胞系后的凋亡情况。

结果

通过显微镜观察、DNA电泳、TUNEL法及流式细胞术检测证实了Ad-rAFP-Mel转染的BEL-7402细胞出现形态学改变及凋亡。Ad-rAFP-Mel组DNA电泳可见DNA梯状条带。TUNEL法检测Ad-rAFP-Mel组、Ad-rAFP组及对照组BEL-7402细胞凋亡率分别为（21.5±2.4）%、（10.5±4.4）%和（3.0±1.4）%（F = 38.0，P < 0.05）；流式细胞术检测凋亡率分别为（7.3±0.5）%、（3.9±0.1）%和（0.8±0.1）%（F = 415.1，P < 0.05）。