一种用于检测核小体核心颗粒中DNA可及性的“一锅法”检测方法。
A 'one-pot' assay for the accessibility of DNA in a nucleosome core particle.
作者信息
Wu Chenyi, Travers Andrew
机构信息
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
出版信息
Nucleic Acids Res. 2004 Aug 25;32(15):e122. doi: 10.1093/nar/gnh121.
Abstract
The accessibility of nucleosomal DNA to transcription factors and other sequence-specific DNA binding proteins is of importance in the consideration of mechanisms of transcriptional control. Here, we report a simple novel assay which determines this accessibility at eight different rotationally equivalent sites on nucleosomal DNA and shows that linker histones and the chromosomal HMGB proteins, HMG-D and HMG-Z, have opposite effects on the accessibility of nucleosomal DNA. We compare this assay to previously described methods.
摘要
核小体DNA对转录因子和其他序列特异性DNA结合蛋白的可及性在转录控制机制的研究中具有重要意义。在此，我们报告了一种简单的新检测方法，该方法可测定核小体DNA上八个不同旋转等效位点的可及性，并表明连接组蛋白和染色体HMGB蛋白HMG-D和HMG-Z对核小体DNA的可及性具有相反的影响。我们将该检测方法与先前描述的方法进行了比较。
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