SWI/SNF作用的原子力显微镜成像：绘制核小体重塑与滑动图谱。
Atomic force microscopy imaging of SWI/SNF action: mapping the nucleosome remodeling and sliding.
作者信息
Montel Fabien, Fontaine Emeline, St-Jean Philippe, Castelnovo Martin, Faivre-Moskalenko Cendrine
机构信息
Laboratoire Joliot-Curie (CNRS USR 3010) et Laboratoire de Physique (CNRS UMR 5672), Ecole Normale Supérieure de Lyon, 69007 Lyon, France.
出版信息
Biophys J. 2007 Jul 15;93(2):566-78. doi: 10.1529/biophysj.107.105569. Epub 2007 Apr 27.
Abstract
We propose a combined experimental (atomic force microscopy) and theoretical study of the structural and dynamical properties of nucleosomes. In contrast to biochemical approaches, this method allows us to determine simultaneously the DNA-complexed length distribution and nucleosome position in various contexts. First, we show that differences in the nucleoproteic structure observed between conventional H2A and H2A.Bbd variant nucleosomes induce quantitative changes in the length distribution of DNA-complexed with histones. Then, the sliding action of remodeling complex SWI/SNF is characterized through the evolution of the nucleosome position and wrapped DNA length mapping. Using a linear energetic model for the distribution of DNA-complexed length, we extract the net-wrapping energy of DNA onto the histone octamer and compare it to previous studies.
摘要
我们提出了一项结合实验（原子力显微镜）和理论的研究，以探讨核小体的结构和动力学特性。与生化方法不同，该方法使我们能够在各种情况下同时确定与DNA复合的长度分布和核小体位置。首先，我们表明，在传统H2A和H2A.Bbd变体核小体之间观察到的核蛋白结构差异会导致与组蛋白复合的DNA长度分布发生定量变化。然后，通过核小体位置的演变和包裹DNA长度图谱来表征重塑复合物SWI/SNF的滑动作用。使用线性能量模型来描述与DNA复合的长度分布，我们提取了DNA在组蛋白八聚体上的净包裹能量，并将其与先前的研究进行比较。
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