Douet-Guilbert Nathalie, Morel Frédéric, Le Charpentier Tiffen, Le Bris Marie-Josée, Herry Angèle, Morice Patrick, Bourquard Pascal, Abgrall Jean François, Berthou Christian, De Braekeleer Marc
Laboratoire d'Histologie, Embryologie et Cytogénétique, Faculté de Médecine et des Sciences de la Santé, Université de Bretagne Occidentale, Brest, France.
Anticancer Res. 2004 Jul-Aug;24(4):2535-9.
Several attempts have been made to determine whether interphase fluorescence in situ hybridization (I-FISH) on bone marrow or peripheral blood specimens is a good alternative to conventional cytogenetics (CC) in calculating the residual proportion of Philadelphia (Ph) chromosome-positive cells during treatment follow-up of patients with chronic myeloid leukemia.
Nineteen patients were selected for I-FISH follow-up compared to CC. All samples were also classified into 4 groups according to the percentage of residual Ph chromosome-positive metaphases analyzed in CC. I-FISH was performed using the LSI bcr/abl dual ES color probe (Vysis).
A high correlation was observed between the frequency of Ph chromosome-positive cells, assessed by CC and I-FISH (p<0.001). A high correlation was found between CC and I-FISH for 12 patients, but not for the remaining 7. Applying the same classification for I-FISH did not show a good relationship between the two techniques (p<0.001).
Dual color I-FISH is a reliable method to monitor the size of the Ph chromosome-positive clone in bone marrow of treated CML patients. However, it has to be complementary to conventional cytogenetics because it cannot detect the emergence of other chromosomal abnormalities in Ph chromosome-positive or -negative cells.
已经进行了多项尝试，以确定在慢性髓性白血病患者的治疗随访期间，对骨髓或外周血标本进行间期荧光原位杂交（I-FISH）是否是计算费城（Ph）染色体阳性细胞残留比例的传统细胞遗传学（CC）的良好替代方法。
选择19例患者进行I-FISH随访，并与CC进行比较。所有样本还根据CC分析的残留Ph染色体阳性中期细胞的百分比分为4组。使用LSI bcr/abl双色ES探针（Vysis）进行I-FISH。
通过CC和I-FISH评估的Ph染色体阳性细胞频率之间观察到高度相关性（p<0.001）。12例患者的CC和I-FISH之间发现高度相关性，但其余7例患者则不然。对I-FISH应用相同的分类并未显示两种技术之间的良好关系（p<0.001）。
双色I-FISH是监测接受治疗的慢性髓性白血病患者骨髓中Ph染色体阳性克隆大小的可靠方法。然而，它必须与传统细胞遗传学互补，因为它无法检测Ph染色体阳性或阴性细胞中其他染色体异常的出现。
