Tian Tian, Qian Si-xuan, Xu Wei, Qiu Hai-rong, Wang Rong, Qiao Chun, Hong Ming, Zhang Jian-fu, Miao Kou-rong, Li Jian-yong
Department of Hematology, the First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, 210029 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Feb;26(1):78-81. doi: 10.3760/cma.j.issn.1003-9406.2009.01.018.
To investigate the incidence of Philadelphia chromosome (Ph) in adult B-lineage acute lymphoblastic leukemia (B-ALL).
One hundred and twelve adult patients with previously untreated B-ALL were prospectively investigated by interphase dual-color dual-fusion fluorescence in situ hybridization (DD-FISH) with two-color break apart probe BCR-ABL and the results were compared with that of conventional cytogenetics (CC).
The incidence of Ph chromosome was 17.98% (16/89) and 31.25% (35/112) by CC and DD-FISH, respectively. The mean positive rate of Ph+cells by FISH was 66.23% (ranging 18.5%-99%). Of the 35 Ph+ALL patients by FISH, 25 were successfully karyotyped by CC which included 5 normal karyotypes, 20 abnormal karyotypes including 16 Ph chromosome and 13 complex abnormalities.
The incidence of Ph chromosome was 31.25% in adult with B-ALL. DD-FISH with BCR-ABL probe provides a powerful technique for the diagnosis of Ph+B-ALL. It is an important supplement to the CC analysis. DD-FISH technique should be used as a routine method for the diagnosis for adult acute B-ALL.
探讨成人B淋巴细胞系急性淋巴细胞白血病（B-ALL）中费城染色体（Ph）的发生率。
采用双色分离探针BCR-ABL的间期双色双融合荧光原位杂交（DD-FISH）技术，对112例未经治疗的成人B-ALL患者进行前瞻性研究，并将结果与传统细胞遗传学（CC）结果进行比较。
CC法和DD-FISH法检测Ph染色体的发生率分别为17.98%（16/89）和31.25%（35/112）。FISH检测Ph+细胞的平均阳性率为66.23%（范围为18.5%-99%）。在35例FISH检测为Ph+的ALL患者中，25例通过CC成功进行了核型分析，其中5例核型正常，20例核型异常，包括16例Ph染色体和13例复杂异常。
成人B-ALL中Ph染色体的发生率为31.25%。采用BCR-ABL探针的DD-FISH为诊断Ph+B-ALL提供了一种强大的技术。它是CC分析的重要补充。DD-FISH技术应作为成人急性B-ALL诊断的常规方法。
