Liebler Eduard K, Diederichsen Ulf
Institut für Organische und Biomolekulare Chemie, Universität Göttingen, Tammannstrasse 2, D-37077 Göttingen, Germany.
Org Lett. 2004 Aug 19;6(17):2893-6. doi: 10.1021/ol049016a.
The design and synthesis of a small peptide that mimics the integration host factor (IHF), a major nucleoid-associated protein, is reported. IHF induces DNA compaction by sequence-specific binding that leads to significant bending of the DNA double strand. In a modular approach a small L-lysine dendrimer responsible for nonspecific charge-charge interactions was linked to a cyclopeptide. The latter was designed for specific DNA recognition in the minor groove followed by bending of the double strand.
报道了一种模拟整合宿主因子（IHF，一种主要的类核相关蛋白）的小肽的设计与合成。IHF通过序列特异性结合诱导DNA压缩，这种结合会导致DNA双链发生显著弯曲。采用模块化方法，将负责非特异性电荷-电荷相互作用的小L-赖氨酸树枝状聚合物与一种环肽相连。后者被设计用于在小沟中特异性识别DNA，随后使双链弯曲。
