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相似文献

1
Effects of adenylyl imidodiphosphate, a nonhydrolyzable adenosine triphosphate analog, on reactivated and rigor wave sea urchin sperm.腺苷酰亚胺二磷酸（一种不可水解的三磷酸腺苷类似物）对再活化和强直波海胆精子的影响。
J Cell Biol. 1978 Dec;79(3):827-32. doi: 10.1083/jcb.79.3.827.
2
Mechanochemical coupling in the relaxation of rigor-wave sea urchin sperm flagella.僵硬波海胆精子鞭毛松弛过程中的机械化学偶联
J Cell Biol. 1982 Mar;92(3):733-41. doi: 10.1083/jcb.92.3.733.
3
Study of the mechanism of vanadate inhibition of the dynein cross-bridge cycle in sea urchin sperm flagella.钒酸盐对海胆精子鞭毛中动力蛋白横桥循环抑制机制的研究。
J Cell Biol. 1979 Jul;82(1):291-8. doi: 10.1083/jcb.82.1.291.
4
Resolution of the competitive inhibitory effects of lithium and AMPPNP on the beat frequency of ATP-reactivated, demembranated, sea urchin sperm flagella.锂和AMPPNP对ATP激活的、去膜的海胆精子鞭毛搏动频率的竞争性抑制作用的消除。
J Muscle Res Cell Motil. 1985 Aug;6(4):507-12. doi: 10.1007/BF00712586.
5
Effects of AMPPNP and vanadate on the mechanochemical crossbridge cycle in flagella.腺苷-5'-（β，γ-亚甲基）三磷酸和钒酸盐对鞭毛机械化学横桥循环的影响。
J Muscle Res Cell Motil. 1981 Mar;2(1):131-40. doi: 10.1007/BF00712064.
6
Anthraniloyl ATP, a fluorescent analog of ATP, as a substrate for dynein ATPase and flagellar motility.邻氨基苯甲酰基ATP，一种ATP的荧光类似物，作为动力蛋白ATP酶和鞭毛运动的底物。
Arch Biochem Biophys. 1989 Oct;274(1):209-15. doi: 10.1016/0003-9861(89)90432-3.
7
Phosphorothioate analogs of ATP as the substrates of dynein and ciliary or flagellar movement.作为动力蛋白以及纤毛或鞭毛运动底物的ATP硫代磷酸酯类似物。
Eur J Biochem. 1990 Aug 17;191(3):543-50. doi: 10.1111/j.1432-1033.1990.tb19155.x.
8
Inhibition by ATP and activation by ADP in the regulation of flagellar movement in sea urchin sperm.ATP对海胆精子鞭毛运动调节的抑制作用及ADP的激活作用。
Cell Motil Cytoskeleton. 2007 Oct;64(10):777-93. doi: 10.1002/cm.20222.
9
Properties of flagellar "rigor waves" formed by abrupt removal of adenosine triphosphate from actively swimming sea urchin sperm.通过从活跃游动的海胆精子中突然去除三磷酸腺苷而形成的鞭毛“强直波”的特性。
J Cell Biol. 1974 Dec;63(3):970-85. doi: 10.1083/jcb.63.3.970.
10
Study of the properties of MgATP2--induced stationary bends in demembranated sea urchin sperm.去膜海胆精子中MgATP2诱导的固定弯曲特性研究。
Cell Motil. 1985;5(3):209-24. doi: 10.1002/cm.970050304.

引用本文的文献

1
Effects of AMPPNP and vanadate on the mechanochemical crossbridge cycle in flagella.腺苷-5'-（β，γ-亚甲基）三磷酸和钒酸盐对鞭毛机械化学横桥循环的影响。
J Muscle Res Cell Motil. 1981 Mar;2(1):131-40. doi: 10.1007/BF00712064.
2
erythro-9-[3-(2-Hydroxynonyl)]adenine is an inhibitor of sperm motility that blocks dynein ATPase and protein carboxylmethylase activities.赤藓红-9-[3-(2-羟基壬基)]腺嘌呤是一种精子活力抑制剂，可阻断动力蛋白ATP酶和蛋白质羧甲基酶的活性。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1033-6. doi: 10.1073/pnas.78.2.1033.
3
Calmodulin confers calcium sensitivity on ciliary dynein ATPase.钙调蛋白赋予纤毛动力蛋白ATP酶钙敏感性。
J Cell Biol. 1980 Nov;87(2 Pt 1):386-97. doi: 10.1083/jcb.87.2.386.
4
Interactions of dynein arms with b subfibers of Tetrahymena cilia: quantitation of the effects of magnesium and adenosine triphosphate.动力蛋白臂与四膜虫纤毛b亚纤维的相互作用：镁离子和三磷酸腺苷作用的定量分析
J Cell Biol. 1980 Oct;87(1):84-97. doi: 10.1083/jcb.87.1.84.
5
Mechanochemical coupling in the relaxation of rigor-wave sea urchin sperm flagella.僵硬波海胆精子鞭毛松弛过程中的机械化学偶联
J Cell Biol. 1982 Mar;92(3):733-41. doi: 10.1083/jcb.92.3.733.
6
Resolution of the competitive inhibitory effects of lithium and AMPPNP on the beat frequency of ATP-reactivated, demembranated, sea urchin sperm flagella.锂和AMPPNP对ATP激活的、去膜的海胆精子鞭毛搏动频率的竞争性抑制作用的消除。
J Muscle Res Cell Motil. 1985 Aug;6(4):507-12. doi: 10.1007/BF00712586.
7
The motile beta/IC1 subunit of sea urchin sperm outer arm dynein does not form a rigor bond.海胆精子外臂动力蛋白的可移动β/IC1亚基不会形成僵硬键。
J Cell Biol. 1992 Sep;118(5):1177-88. doi: 10.1083/jcb.118.5.1177.
8
Study of the mechanism of vanadate inhibition of the dynein cross-bridge cycle in sea urchin sperm flagella.钒酸盐对海胆精子鞭毛中动力蛋白横桥循环抑制机制的研究。
J Cell Biol. 1979 Jul;82(1):291-8. doi: 10.1083/jcb.82.1.291.

本文引用的文献

1
Dynein: A Protein with Adenosine Triphosphatase Activity from Cilia.动力蛋白：纤毛中的一种具有三磷酸腺苷酶活性的蛋白质。
Science. 1965 Jul 23;149(3682):424-6. doi: 10.1126/science.149.3682.424.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Electron microscopy of the sperm tail; results obtained with a new fixative.精子尾部的电子显微镜检查；用一种新型固定剂获得的结果。
J Biophys Biochem Cytol. 1959 Mar 25;5(2):269-78. doi: 10.1083/jcb.5.2.269.
4
Adenylyl imidodiphosphate, an adenosine triphosphate analog containing a P--N--P linkage.腺苷亚氨基二磷酸，一种含有P-N-P键的三磷酸腺苷类似物。
Biochemistry. 1971 Jun 22;10(13):2484-9. doi: 10.1021/bi00789a009.
5
Studies on flagellar ATPase from sea urchin spermatozoa. I. Purification and some properties of the enzyme.海胆精子鞭毛ATP酶的研究。I. 酶的纯化及某些性质
Biochim Biophys Acta. 1972 Jan 21;256(1):142-55. doi: 10.1016/0005-2728(72)90169-7.
6
Interaction of P--N--P and P--C--P analogs of adenosine triphosphate with heavy meromyosin, myosin, and actomyosin.三磷酸腺苷的P--N--P和P--C--P类似物与重酶解肌球蛋白、肌球蛋白和肌动球蛋白的相互作用。
Biochemistry. 1971 Jun 22;10(13):2490-6. doi: 10.1021/bi00789a010.
7
Effects of viscosity and ATP concentration on the movement of reactivated sea-urchin sperm flagella.粘度和ATP浓度对再活化海胆精子鞭毛运动的影响。
J Exp Biol. 1975 Jun;62(3):701-19. doi: 10.1242/jeb.62.3.701.
8
The role of the bound nucleotide in the polymerization of actin.结合核苷酸在肌动蛋白聚合中的作用。
Biochemistry. 1975 Jul 15;14(14):3250-6. doi: 10.1021/bi00685a035.
9
Computer simulation of flagellar movement. V. oscillation of cross-bridge models with an ATP-concentration-dependent rate function.鞭毛运动的计算机模拟。V. 具有ATP浓度依赖性速率函数的横桥模型的振荡
J Mechanochem Cell Motil. 1977 Sep;4(3):205-32.
10
Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components.缺乏辐条和中央微管的衣藻鞭毛突变体。特定轴丝成分的结构、组成和功能。
J Cell Biol. 1978 Mar;76(3):729-47. doi: 10.1083/jcb.76.3.729.

腺苷酰亚胺二磷酸（一种不可水解的三磷酸腺苷类似物）对再活化和强直波海胆精子的影响。

Effects of adenylyl imidodiphosphate, a nonhydrolyzable adenosine triphosphate analog, on reactivated and rigor wave sea urchin sperm.

作者信息

Penningroth S M, Witman G B

出版信息

J Cell Biol. 1978 Dec;79(3):827-32. doi: 10.1083/jcb.79.3.827.

DOI:10.1083/jcb.79.3.827
PMID:153347
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2110271/
Abstract

A nonhydrolyzable ATP analog, adenylyl imidodiphosphate (AMP-PNP), has been used to study the role of ATP binding in flagellar motility. Sea urchin sperm of Lytechinus pictus were demembranated, reactivated, and locked in "rigor waves" by a modification of the method of Gibbons and Gibbons (11). Rigor wave sperm relaxed within 2 min after addition of 4 micrometer ATP, and reactivated upon addition of 10-12 micrometer ATP. The beat frequency of the reactivated sperm varied with ATP concentration according to Michaelis-Menten kinetics ("Km" = 0.24 mM; "Vmax" = 44 Hz) and was competitively inhibited by AMP-PNP (Ki" approximately to 8.1 mM). Rigor wave sperm were completely relaxed (straightened) within 2 min by AMP-PNP at concentrations of 2-4 mM. The possibilities that relaxation in AMP-PNP was a result of ATP contamination, AMP-PNP hydrolysis, or lowering of the free Mg++ concentration were conclusively ruled out. The results suggest that dynein cross-bridge release is dependent upon ATP binding but not hydrolysis.

摘要

一种不可水解的ATP类似物，腺苷酰亚胺二磷酸（AMP-PNP），已被用于研究ATP结合在鞭毛运动中的作用。通过对吉本斯和吉本斯（11）方法的改进，对美西刺海胆精子进行去膜、重新激活，并使其锁定在“僵直波”状态。在加入4微摩尔ATP后，僵直波精子在2分钟内松弛，并在加入10 - 12微摩尔ATP后重新激活。重新激活的精子的摆动频率根据米氏动力学随ATP浓度变化（“Km” = 0.24毫摩尔；“Vmax” = 44赫兹），并被AMP-PNP竞争性抑制（“Ki ”约为8.1毫摩尔）。在浓度为2 - 4毫摩尔的AMP-PNP作用下，僵直波精子在2分钟内完全松弛（伸直）。AMP-PNP导致松弛是由于ATP污染、AMP-PNP水解或游离Mg++浓度降低的可能性被完全排除。结果表明，动力蛋白横桥的释放依赖于ATP结合而非水解。