基于recA基因序列分析瑞士广义伯氏疏螺旋体基因种内的多样性

Diversity within Borrelia burgdorferi sensu lato genospecies in Switzerland by recA gene sequence.

作者信息

Casati Simona, Bernasconi Marco V, Gern Lise, Piffaretti Jean-Claude

机构信息

Istituto Cantonale di Microbiologia, Via Mirasole 22A, 6500 Bellinzona, Switzerland.

出版信息

FEMS Microbiol Lett. 2004 Sep 1;238(1):115-23. doi: 10.1016/j.femsle.2004.07.025.

DOI:10.1016/j.femsle.2004.07.025

PMID:15336411

Abstract

A total of 874 Ixodes ricinus ticks were collected in Switzerland to investigate the genetic diversity of the Borrelia population. We integrated to the RT-PCR method the DNA sequence analysis of a 162-bp fragment of the recA gene. Five genospecies were detected: Borrelia afzelii, Borrelia burgdorferi s.s., Borrelia garinii, Borrelia valaisiana, and Borrelia lusitaniae. A heterogeneous distribution was observed within the B. burgdorferi s.l. genospecies. The most prevalent and diverse genospecies found in Switzerland was Borrelia afzelii, which might suggest a rapid evolution of this genospecies.

摘要

在瑞士共采集了874只蓖麻硬蜱，以调查伯氏疏螺旋体种群的遗传多样性。我们将recA基因162bp片段的DNA序列分析整合到RT-PCR方法中。检测到五种基因种：阿氏疏螺旋体、狭义伯氏疏螺旋体、伽氏疏螺旋体、瓦莱州疏螺旋体和卢西塔尼亚疏螺旋体。在广义伯氏疏螺旋体基因种内观察到异质分布。在瑞士发现的最普遍和最多样化的基因种是阿氏疏螺旋体，这可能表明该基因种的快速进化。