从甜菜细胞壁中分离与阿拉伯聚糖酯键相连的二阿魏酸桥。
Isolation of diferulic bridges ester-linked to arabinan in sugar beet cell walls.
作者信息
Levigne Sébastien, Ralet Marie-Christine, Quéméner Bernard, Thibault Jean-François
机构信息
Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, INRA, rue de la Géraudière, B.P. 71627, F-44316 Nantes Cedex 3, France.
出版信息
Carbohydr Res. 2004 Sep 13;339(13):2315-9. doi: 10.1016/j.carres.2004.07.006.
After degradation of sugar beet cell walls with Driselase and fractionation of the solubilised products by hydrophobic interaction chromatography, a dehydrodiferuloylated oligoarabinan was isolated. Its structure was assigned to two dimers of (1-->5)-linked arabinose units esterified by a central 8-O-4' ferulic dimer. These results provide the first direct evidence that pectic arabinans in sugar beet cell walls may be covalently cross-linked through dehydrodiferulates.
在用里斯酶降解甜菜细胞壁并通过疏水相互作用色谱法对溶解产物进行分级分离后，分离出了一种脱氢二阿魏酰化低聚阿拉伯聚糖。其结构被确定为由（1→5）连接的阿拉伯糖单元的两个二聚体组成，这些单元由一个中心的8-O-4'阿魏酸二聚体酯化。这些结果提供了首个直接证据，表明甜菜细胞壁中的果胶阿拉伯聚糖可能通过脱氢二阿魏酸共价交联。
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