Wageningen University, Laboratory of Food Chemistry, Wageningen, The Netherlands.
Carbohydr Res. 2010 Jun 16;345(9):1180-9. doi: 10.1016/j.carres.2010.03.042. Epub 2010 Apr 4.
Sugar beet arabinan consists of an alpha-(1,5)-linked backbone of L-arabinosyl residues, which can be either single or double substituted with alpha-(1,2)- and/or alpha-(1,3)-linked L-arabinosyl residues. Neutral branched arabino-oligosaccharides were isolated from sugar beet arabinan by enzymatic degradation with mixtures of pure and well-defined arabinohydrolases from Chrysosporium lucknowense followed by fractionation based on size and analysis by MALDI-TOF MS and HPAEC. Using NMR analysis, two main series of branched arabino-oligosaccharides have been identified, both having an alpha-(1,5)-linked backbone of L-arabinosyl residues. One series carries single substituted alpha-(1,3)-linked L-arabinosyl residues at the backbone, whereas the other series consists of a double substituted alpha-(1,2,3,5)-linked arabinan structure within the molecule. The structures of eight such branched arabino-oligosaccharides were established.
糖甜菜阿拉伯聚糖由 L-阿拉伯糖基残基的α-(1,5)-连接主链组成，这些残基可以单取代或双取代为α-(1,2)-和/或α-(1,3)-连接的 L-阿拉伯糖基残基。中性支链阿拉伯寡糖通过用来自 Chrysosporium lucknowense 的纯且定义明确的阿拉伯水解酶混合物对糖甜菜阿拉伯聚糖进行酶促降解，然后根据大小进行分级，并通过 MALDI-TOF MS 和 HPAEC 进行分析，从糖甜菜阿拉伯聚糖中分离出来。通过 NMR 分析，已经确定了两种主要的支链阿拉伯寡糖系列，它们都具有 L-阿拉伯糖基残基的α-(1,5)-连接主链。一个系列在主链上带有单取代的α-(1,3)-连接的 L-阿拉伯糖基残基，而另一个系列由分子内的双取代的α-(1,2,3,5)-连接的阿拉伯聚糖结构组成。确定了 8 种此类支链阿拉伯寡糖的结构。
