Anastasopoulos E, Reclos G J, Baxevanis C N, Gritzapis A D, Tsilivakos V, Panagiotopoulos N, Fotiou S, Missitzis I, Karydas I, Papamichail M
Department of Immunology, Hellenic Anticancer Institute, Athens, Greece.
Anticancer Res. 1992 Mar-Apr;12(2):489-94.
T cells proliferate in response to autologous monocytes in the autologous mixed lymphocyte reaction (AMLR). AMLR was found to be impaired in patients with advanced cancer (stages III and IV), whereas normal values were found in the early stages of the disease (stages I and II). Peripheral T lymphocytes from patients with advanced stages also exhibited a decreased ability to produce Interleukin-2 (IL-2) during an AMLR response, whereas production of IL-2 by T cells in stages I and II was comparable to that of normal donors. The impaired IL-2 production by T lymphocytes in the AMLR was associated with high concentrations of soluble interleukin-2 receptor (sIL-2R) in culture supernatants and reduced expression of membrane-bound interleukin-2 receptors (IL-2R) on the same AMLR-activated T lymphocytes. These abnormalities in T cells from cancer patients were demonstrated to be associated with dysfunctions of autologous monocytes. Thus monocytes from patients with advanced cancer exhibited diminished expression of HLA-DR antigens and produced low levels of Interleukin-1 beta (IL-1 beta) and Tumor Necrosis Factor a (TNFa). No changes were detected in the expression of HLA-A, -B, -C antigens. The results presented here demonstrate that decreased in vitro T cell responses may be attributed to monocyte dysfunctions in these patients and provide new information for a better understanding of the impaired T cell function in cancer patients.
在自体混合淋巴细胞反应（AMLR）中，T细胞会对自体单核细胞产生增殖反应。研究发现，晚期癌症患者（III期和IV期）的AMLR受损，而在疾病早期（I期和II期）则为正常水平。晚期患者的外周血T淋巴细胞在AMLR反应过程中产生白细胞介素-2（IL-2）的能力也有所下降，而I期和II期患者T细胞产生IL-2的能力与正常供体相当。AMLR中T淋巴细胞产生IL-2的能力受损与培养上清液中高浓度的可溶性白细胞介素-2受体（sIL-2R）以及同一AMLR激活的T淋巴细胞上膜结合白细胞介素-2受体（IL-2R）表达降低有关。癌症患者T细胞的这些异常表现被证明与自体单核细胞功能障碍有关。因此，晚期癌症患者的单核细胞HLA-DR抗原表达减少，白细胞介素-1β（IL-1β）和肿瘤坏死因子α（TNFα）产生水平较低。而HLA-A、-B、-C抗原的表达未检测到变化。此处呈现的结果表明，这些患者体外T细胞反应降低可能归因于单核细胞功能障碍，并为更好地理解癌症患者T细胞功能受损提供了新信息。
