Tsai C L, Liu T K
Department of Orthopaedic Surgery, School of Medicine, National Taiwan University, Taipei, Republic of China.
Tohoku J Exp Med. 1992 Mar;166(3):289-96. doi: 10.1620/tjem.166.289.
The changes and the possible roles of splenic T lymphocyte subpopulations in high-responder Long-Evans (LE) rats and low-responder Sprague-Dawley (SD) rats with induced adjuvant arthritis (AA) were investigated. Total splenic T lymphocytes of both strains did not change significantly after the adjuvant injection. However, LE rats exhibited an increase in percentage of helper T cells and helper/suppressor T cell ratio, whereas SD rats expressed a decrease in the ratio with concomitant increase of suppressor T cells. This observation suggested that an imbalance of T lymphocyte ratio not only existed in the peripheral blood as reported by other investigators, but also existed in the spleen of rats with AA. In vitro measurement of phytohemagglutinin (PHA) responses of splenic T lymphocytes revealed an increased PHA response in LE but a markedly decreased PHA response in SD. Both LE and SD rats showed decreased response to concanavalin A (Con A) stimulation. We concluded that PHA response, as an index of helper T cell function, coincided with the development of AA and may be responsible for the immunoregulation of the disease. The increased proliferation of suppressor T cell in SD rats may also be significant in regulating the immune response to AA.
研究了高反应性长 Evans（LE）大鼠和低反应性斯普拉格-道利（SD）大鼠在诱导佐剂性关节炎（AA）时脾 T 淋巴细胞亚群的变化及其可能的作用。两种品系大鼠在注射佐剂后脾 T 淋巴细胞总数均无明显变化。然而，LE 大鼠辅助性 T 细胞百分比和辅助性/抑制性 T 细胞比值增加，而 SD 大鼠该比值降低，同时抑制性 T 细胞增加。这一观察结果表明，T 淋巴细胞比例失衡不仅如其他研究者报道的那样存在于外周血中，也存在于患 AA 的大鼠脾脏中。体外测量脾 T 淋巴细胞对植物血凝素（PHA）的反应发现，LE 大鼠的 PHA 反应增强，而 SD 大鼠的 PHA 反应明显降低。LE 和 SD 大鼠对刀豆蛋白 A（Con A）刺激的反应均降低。我们得出结论，PHA 反应作为辅助性 T 细胞功能的指标，与 AA 的发展一致，可能负责该疾病的免疫调节。SD 大鼠中抑制性 T 细胞增殖增加在调节对 AA 的免疫反应中可能也很重要。
