Rodríguez-Palmero M, Pelegrí C, Ferri M J, Castell M, Franch A, Castellote C
Department de Fisiologia-Divisió IV, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Inflammation. 1999 Apr;23(2):153-65. doi: 10.1023/a:1020293012793.
The aim of this study was to analyze potential imbalances in lymphocyte populations from regional lymph nodes (LN) and spleen occurring before the development of the outer inflammation of adjuvant arthritis (AA). Percentages and absolute numbers of CD5+, CD4+, CD8+, Ig+, I-A+, NKR-P1+ and TCRgammadelta+ cells were determined. No differences in percentages of gammadelta T or NK cells were found either in LN or spleen, thus ruling out an important role of these minor subpopulations in these early stages of AA. While no significant lymphocyte imbalances were observed in spleen, an increase in the percentage of B lymphocytes was found in regional LN. Moreover, a high proliferation of CD8+ cells was observed when measuring absolute numbers of LN lymphocytes, thus producing an imbalance in the CD4/CD8 ratio at very early stages of the inflammatory process. These findings suggest a role for CD8+ and B lymphocytes in the latency period of AA at the LN level. Our results indicate a primary role for lymph nodes in initiating the inflammation of AA, whereas cells from the spleen probably play a secondary role.
本研究的目的是分析佐剂性关节炎（AA）外周炎症发生之前，区域淋巴结（LN）和脾脏中淋巴细胞群体的潜在失衡情况。测定了CD5+、CD4+、CD8+、Ig+、I-A+、NKR-P1+和TCRγδ+细胞的百分比和绝对数量。在LN或脾脏中，均未发现γδ T细胞或NK细胞百分比存在差异，因此排除了这些次要亚群在AA早期阶段的重要作用。虽然在脾脏中未观察到明显的淋巴细胞失衡，但在区域LN中发现B淋巴细胞百分比增加。此外，在测量LN淋巴细胞绝对数量时，观察到CD8+细胞的高增殖，从而在炎症过程的非常早期阶段导致CD4/CD8比值失衡。这些发现表明CD8+和B淋巴细胞在LN水平的AA潜伏期发挥作用。我们的结果表明淋巴结在引发AA炎症中起主要作用，而脾脏中的细胞可能起次要作用。
