Matroule Jean-Yves, Lam Hubert, Burnette Dylan T, Jacobs-Wagner Christine
Department of Molecular, Cellular, and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520, USA.
Cell. 2004 Sep 3;118(5):579-90. doi: 10.1016/j.cell.2004.08.019.
For successful generation of different cell types by asymmetric cell division, cell differentiation should be initiated only after completion of division. Here, we describe a control mechanism by which Caulobacter couples the initiation of a developmental program to the completion of cytokinesis. Genetic evidence indicates that localization of the signaling protein DivK at the flagellated pole prevents premature initiation of development. Photobleaching and FRET experiments show that polar localization of DivK is dynamic with rapid pole-to-pole shuttling of diffusible DivK generated by the localized activities of PleC phosphatase and DivJ kinase at opposite poles. This shuttling is interrupted upon completion of cytokinesis by the segregation of PleC and DivJ to different daughter cells, resulting in disruption of DivK localization at the flagellated pole and subsequent initiation of development in the flagellated progeny. Thus, dynamic polar localization of a diffusible protein provides a control mechanism that monitors cytokinesis to regulate development.
为了通过不对称细胞分裂成功产生不同的细胞类型，细胞分化应仅在分裂完成后启动。在这里，我们描述了一种控制机制，通过该机制，柄杆菌将发育程序的启动与胞质分裂的完成联系起来。遗传证据表明，信号蛋白DivK在鞭毛极的定位可防止发育过早启动。光漂白和荧光共振能量转移实验表明，DivK的极性定位是动态的，由相反极的PleC磷酸酶和DivJ激酶的局部活性产生的可扩散DivK在极间快速穿梭。胞质分裂完成后，PleC和DivJ分离到不同的子细胞中，这种穿梭被打断，导致DivK在鞭毛极的定位被破坏，随后鞭毛后代开始发育。因此，一种可扩散蛋白的动态极性定位提供了一种监测胞质分裂以调节发育的控制机制。
