Gao Xiu Kun, Nakamura Nobuaki, Fuseda Kazutoshi, Tanaka Hiroyuki, Inagaki Naoki, Nagai Hiroichi
Department of Pharmacology, Gifu Pharmaceutical University, 5-6-1 Mitahorahigashi, Gifu 502-8585, Japan.
Biol Pharm Bull. 2004 Sep;27(9):1376-81. doi: 10.1248/bpb.27.1376.
Mite antigen has been suggested to play important roles in the onset and/or development of atopic dermatitis, and mite antigen-induced dermatitis models appear beneficial for the basic study of atopic dermatitis. In the present study therefore, we attempted to establish an allergic dermatitis model in mice using Dermatophagoides farinae crude extract as an antigen. Mite antigen solution at a concentration of 1 or 10 mg/ml was painted 5 times repeatedly at an interval of 7 d onto the ear of NC/Nga or BALB/c mice with or without simultaneous tape-stripping. Apparent biphasic ear swelling was observed after the 4th and 5th antigen applications in both strains of mice treated with 10 mg/ml of antigen solution. Thickening of the epidermis, fibrosis of the dermis, and the accumulation of inflammatory cells were also observed after the 5th application. The inflammatory changes were more evident in NC/Nga mice than in BALB/c mice and potentiated by tape-stripping. The ear swelling was accompanied by increased serum IgE, increased expression of interleukin-4 mRNA and decreased expression of interferon-gamma mRNA in cervical lymph nodes and ears. These results indicate that ear swelling caused by repeated mite antigen application with simultaneous tape-stripping has a Th2-dominant background and that the inflammatory responses are expressed more potently in NC/Nga mice than in BALB/c mice. The dermatitis caused by mite antigen in NC/Nga mice appears to be a useful model for the basic study of atopic dermatitis.
已有人提出螨抗原在特应性皮炎的发病和/或发展中起重要作用，并且螨抗原诱导的皮炎模型似乎有利于特应性皮炎的基础研究。因此，在本研究中，我们试图用粉尘螨粗提物作为抗原在小鼠中建立过敏性皮炎模型。将浓度为1或10mg/ml的螨抗原溶液以7天的间隔重复涂抹5次到有或没有同时进行胶带剥离的NC/Nga或BALB/c小鼠的耳部。在用10mg/ml抗原溶液处理的两种品系小鼠中，在第4次和第5次应用抗原后观察到明显的双相耳部肿胀。在第5次应用后还观察到表皮增厚、真皮纤维化和炎症细胞积聚。炎症变化在NC/Nga小鼠中比在BALB/c小鼠中更明显，并因胶带剥离而增强。耳部肿胀伴随着血清IgE增加、颈淋巴结和耳部白细胞介素-4 mRNA表达增加以及干扰素-γ mRNA表达减少。这些结果表明，同时进行胶带剥离反复应用螨抗原引起的耳部肿胀具有以Th2为主的背景，并且炎症反应在NC/Nga小鼠中比在BALB/c小鼠中表达更强烈。NC/Nga小鼠中由螨抗原引起的皮炎似乎是特应性皮炎基础研究的有用模型。
