Department of Pharmacology, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju-si, Gangwon-do 26339, Korea.
Department of Korean Ophthalmology and Otolaryngology and Dermatology, College of Korean Medicine, Sangji University, Wonju, Gangwon-do 26339, Korea.
Nutrients. 2018 Nov 3;10(11):1657. doi: 10.3390/nu10111657.
Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by complex symptoms. To treat AD without adverse effects, alternative therapeutic agents are required. The tubers of L. (Jerusalem artichoke) have been used in folk remedies for diabetes and rheumatism. However, its effect on AD development remains unknown. Therefore, this study examined the inhibitory effect of (HT) on AD skin symptoms using an NC/Nga mouse model and HaCaT keratinocytes. The effect of HT and associated molecular mechanisms were evaluated in Dermatophagoides farina body (Dfb)-induced AD mice and tumor necrosis factor (TNF)-α/interferon (IFN)-γ-stimulated HaCaT keratinocytes by ELISA, western blot, and histological analysis. Topical HT administration attenuated AD skin symptoms in Dfb-induced AD mice, with a significant reduction in the dermatitis score and production of inflammatory mediators. HT also decreased epidermal thickness and mast cell infiltration. Moreover, HT restored filaggrin expression and inhibited adhesion molecules in the mice. These effects were confirmed in vitro. Furthermore, HT suppressed the activation of NF-κB, Akt, and mitogen-activated protein kinase (MAPK) signaling pathways induced by TNF-α/IFN-γ. These results suggest that HT is a potential therapeutic agent or supplement for skin allergic inflammatory diseases such as AD.
特应性皮炎（AD）是一种慢性炎症性皮肤病，具有复杂的症状。为了治疗 AD 而不产生不良反应，需要替代治疗药物。 （洋姜）的块茎已被用于民间疗法来治疗糖尿病和风湿病。然而，其对 AD 发展的影响尚不清楚。因此，本研究使用 NC/Nga 小鼠模型和 HaCaT 角质形成细胞来研究 （HT）对 AD 皮肤症状的抑制作用。通过 ELISA、western blot 和组织学分析评估了 HT 及其相关分子机制在粉尘螨体（Dfb）诱导的 AD 小鼠和 TNF-α/IFN-γ 刺激的 HaCaT 角质形成细胞中的作用。局部 HT 给药可减轻 Dfb 诱导的 AD 小鼠的 AD 皮肤症状，显著降低皮炎评分和炎症介质的产生。HT 还降低了表皮厚度和肥大细胞浸润。此外，HT 恢复了小鼠中的丝聚合蛋白表达并抑制了粘附分子。这些作用在体外得到了证实。此外，HT 抑制了 TNF-α/IFN-γ 诱导的 NF-κB、Akt 和丝裂原激活蛋白激酶（MAPK）信号通路的激活。这些结果表明，HT 是一种有潜力的治疗剂或补充剂，可用于治疗 AD 等皮肤过敏性炎症性疾病。
