[艾滋病病毒酶免疫分析诊断试剂盒检测不同基因型艾滋病病毒抗体的能力]
[Capacity of HIV enzyme immunoassay diagnostic kits to detect antibodies against different genotypes of HIV].
作者信息
Song Ai-jing, Li Xiu-hua, Li Juan, Xu Si-hong, Zhang Feng, Wan Yan-min, Zhang Chun-tao, Wang You-chun
机构信息
Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Dec;17(4):319-21.
OBJECTIVE
To investigate the capacity of commercial HIV enzyme immunoassay (EIA) diagnostic kits to detect antibodies against different genotypes of HIV.
METHODS
HIV RNA was detected with RT-PCR from samples positive for HIV antibody. The purified PCR products were sequenced directly and the genotypes of HIV from samples were analyzed. The samples for each genotype of HIV were diluted and the diluted samples were detected with different HIV EIA diagnostic kits.
RESULTS
All 20 samples positive for HIV antibody were also positive for HIV RNA; 9 of 20 isolates were genotype B, 9 of them were genotype C or CRF BC, 2 of them were CRF AE. The sensitivity of different HIV EIA diagnostic kits to detect antibodies against different genotypes of HIV was not significantly different.
CONCLUSION
The capacity of commercial HIV diagnostic kits to detect antibodies against different HIV genotypes may not be significantly different.
目的
探讨商用HIV酶免疫测定（EIA）诊断试剂盒检测针对不同基因型HIV抗体的能力。
方法
用逆转录-聚合酶链反应（RT-PCR）从HIV抗体阳性样本中检测HIV RNA。对纯化的PCR产物直接进行测序，并分析样本中HIV的基因型。将每种HIV基因型的样本进行稀释，并用不同的HIV EIA诊断试剂盒检测稀释后的样本。
结果
20份HIV抗体阳性样本的HIV RNA均为阳性；20株分离株中9株为B基因型，9株为C基因型或CRF BC，2株为CRF AE。不同HIV EIA诊断试剂盒检测针对不同基因型HIV抗体的敏感性无显著差异。
结论
商用HIV诊断试剂盒检测针对不同HIV基因型抗体的能力可能无显著差异。
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