Rai A, Kumari S, Prabhakaran P K
Microbiology Division, National Institute of Communicable Diseases, Delhi.
J Commun Dis. 1991 Jun;23(2):149-53.
Choice of an ideal, cost-effective and rapid diagnostic test for HIV infection is of immense value in developing countries like India where resources are limited. A number of commercial HIV antibody testing kits are now available with varying sensitivities and specificities. Six different commercial HIV kits namely, Wellcozyme, Flow HIV-TEKG, Abbott HIV EIA, Abbott VIA, Dip-stick EIA and Abbott env/core recombinant EIA were evaluated. Du-Pont Western blot (W.B.) kit was used as gold standard to compare the results. Of the 376 sera from various high-risk individuals screened, Wellcozyme kit yielded 100 per cent concordant results with W.B. Abbott VIA and Abbott env/core also yielded results in confirmation with W.B., excepting the fact that both detected one extra sample positive, which was negative in W.B. Abbott EIA yielded 4 false positive results. Dip-stick kit yielded the maximum number of false positives. The study indicated that 3 kits, namely Wellcozyme, Abbott VIA and Abbott EIA could be used to achieve optimum and acceptable results.
在像印度这样资源有限的发展中国家，选择一种理想、经济高效且快速的艾滋病毒感染诊断检测方法具有巨大价值。现在有许多商业性艾滋病毒抗体检测试剂盒，其灵敏度和特异性各不相同。对六种不同的商业艾滋病毒试剂盒，即Wellcozyme、Flow HIV - TEKG、雅培艾滋病毒酶免疫测定法（EIA）、雅培VIA、试纸条EIA和雅培env/core重组EIA进行了评估。使用杜邦免疫印迹（W.B.）试剂盒作为金标准来比较结果。在对376份来自各类高危个体的血清进行筛查时，Wellcozyme试剂盒与免疫印迹法的结果100%一致。雅培VIA和雅培env/core的结果也与免疫印迹法相符，只是两者都检测出一个额外的阳性样本，而该样本在免疫印迹法中为阴性。雅培EIA产生了4例假阳性结果。试纸条试剂盒产生的假阳性数量最多。该研究表明，Wellcozyme、雅培VIA和雅培EIA这三种试剂盒可用于获得最佳且可接受的结果。
