Noulas Argiris V, Skandalis Spyros S, Feretis Elias, Theocharis Dimitrios A, Karamanos Nikos K
Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras 26110 Patras, Greece.
Biomed Chromatogr. 2004 Sep;18(7):457-61. doi: 10.1002/bmc.336.
The vitreous of all species is composed of essentially the same type of extracellular matrix macromolecules organized to a transparent gel. In this study, the composition and fi ne chemical structure of the glycosaminoglycans (GAGs) in the vitreous gel from sheep and goat were determined and compared with those of human and pig vitreous gels. The results showed that, in all examined species; hyaluronan (HA) was the predominant GAG, whereas chondroitin sulphate (CS) was the minor one. In the vitreous gel of the most relative species, i.e. sheep and goat, higher amounts of both of HA and CS were estimated as compared with pig and human tissues. The distribution of hydrodynamic sizes of HA and CS was significantly differed among different species. All HA preparations consisted of molecules with great variability in hydrodynamic sizes. The relative proportions of the large HA molecules (size >1.8 x 10(6) kDa) were significantly higher in sheep and goat as compared with human and pig vitreous gel. The length of CS chains was also of larger size in sheep and goat (50 and 58 kDa, respectively) than the respective chains in human and pig vitreous gel (38 and 28 kDa, respectively). The sulphation patterns of CS preparations were determined following enzymic treatments, HPLC and capillary electrophoretic analyses. The human vitreous-derived CS chains showed quite different sulphation profile than that of CS isolated from other species, since 4-sulphated disaccharides were identified as the dominant moiety. In conclusion, significant compositional and structural variations between the vitreous matrixes of different species at the GAG level were identified. The functional significance of these species-dependent variations is discussed.
所有物种的玻璃体基本上都由相同类型的细胞外基质大分子组成，并组织成透明凝胶。在本研究中，测定了绵羊和山羊玻璃体凝胶中糖胺聚糖（GAGs）的组成和精细化学结构，并与人类和猪的玻璃体凝胶进行了比较。结果表明，在所有检测的物种中，透明质酸（HA）是主要的GAG，而硫酸软骨素（CS）是次要的。在亲缘关系最密切的物种即绵羊和山羊的玻璃体凝胶中，与猪和人类组织相比，HA和CS的含量估计都更高。不同物种之间，HA和CS流体力学尺寸的分布存在显著差异。所有HA制剂都由流体力学尺寸差异很大的分子组成。与人类和猪的玻璃体凝胶相比，绵羊和山羊玻璃体中大分子HA（尺寸>1.8×10⁶ kDa）的相对比例显著更高。绵羊和山羊玻璃体中CS链的长度（分别为50 kDa和58 kDa）也比人类和猪玻璃体凝胶中相应的链（分别为38 kDa和28 kDa）更大。通过酶处理、高效液相色谱和毛细管电泳分析确定了CS制剂的硫酸化模式。源自人类玻璃体的CS链显示出与从其他物种分离的CS完全不同的硫酸化谱，因为4-硫酸化二糖被确定为主要部分。总之，在GAG水平上，不同物种的玻璃体基质之间存在显著的组成和结构差异。讨论了这些物种依赖性差异的功能意义。
